2. Academic Validation
  3. CpG ODN 1668 as TLR9 agonist mediates humpback grouper (Cromileptes altivelis) antibacterial immune responses

CpG ODN 1668 as TLR9 agonist mediates humpback grouper (Cromileptes altivelis) antibacterial immune responses

  • Fish Shellfish Immunol. 2023 Jul:138:108839. doi: 10.1016/j.fsi.2023.108839.
Xiaojuan Chen 1,  Panpan Zhang 2,  Pengshuo Li 3,  Guotao Wang 2,  Jianlong Li 1,  Ying Wu 1,  Zhenjie Cao 1,  Yongcan Zhou 1,  Yun Sun 4
Affiliations

Affiliations

  • 1 Collaborative Innovation Center of Marine Science and Technology, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, China; Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, College of Marine Science, Hainan University, Haikou, 570228, China.
  • 2 Sanya Nanfan Research Institute, Hainan University, Sanya, 572022, China; Collaborative Innovation Center of Marine Science and Technology, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, China.
  • 3 Sanya Nanfan Research Institute, Hainan University, Sanya, 572022, China; Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, College of Marine Science, Hainan University, Haikou, 570228, China.
  • 4 Sanya Nanfan Research Institute, Hainan University, Sanya, 572022, China; Collaborative Innovation Center of Marine Science and Technology, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, China; Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, College of Marine Science, Hainan University, Haikou, 570228, China. Electronic address: syshui207@126.com.
PMID: 37207883 DOI: 10.1016/j.fsi.2023.108839
Abstract

Cromileptes altivelis (humpback grouper) is the main farmed species in the southern coastal area of China owing to its important economic value. Toll-like Receptor 9 (TLR9) belongs to the Toll-like Receptor (TLR) family and functions as a pattern recognition receptor, recognising unmethylated oligodeoxynucleotides containing the CpG motif (CpG ODNs) in Bacterial and viral genomes, thereby activating host immune response. In this study, the C. altivelis TLR9 (CaTLR9) ligand CpG ODN 1668 was screened and found to significantly enhance the Antibacterial immunity of humpback grouper in vivo and head kidney lymphocytes (HKLs) in vitro. In addition, CpG ODN 1668 also promoted the cell proliferation and immune gene expression of HKLs and strengthened the phagocytosis activity of head kidney macrophages. However, when the CaTLR9 expression was knocked down in the humpback group, the expression levels of TLR9, myeloid differentiation factor 88 (MyD88), tumour necrosis factor-α (TNF-α), interferon γ (IFN-γ), interleukin-1β (IL-1β), IL-6, and IL-8 were significantly reduced, and the Antibacterial immune effects induced by CpG ODN 1668 were mostly abolished. Therefore, CpG ODN 1668 induced Antibacterial immune responses in a CaTLR9-dependent pathway. These results enhance the knowledge of the Antibacterial immunity of fish TLR signalling pathways and have important implications for exploring natural Antibacterial molecules in fish.

Keywords

Antibacterial immunity; CaTLR9; CpG ODN 1668; Cromileptes altivelis.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z