2. Academic Validation
  3. Mitotic defects lead to unreduced sperm formation in cdk1-/- mutants

Mitotic defects lead to unreduced sperm formation in cdk1-/- mutants

  • Int J Biol Macromol. 2023 Jun 2;125171. doi: 10.1016/j.ijbiomac.2023.125171.
Yunbang Zhang 1,  Meiqi Lv 2,  Hanjun Jiang 2,  Hui Li 2,  Rongyun Li 2,  Chuang Yang 2,  Yuwei Huang 2,  He Zhou 3,  Yihui Mei 2,  Jian Gao 4,  Xiaojuan Cao 5
Affiliations

Affiliations

  • 1 College of Fisheries, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China; Hubei Provincial Engineering Laboratory for Pond Aquaculture, Hubei, China.
  • 2 College of Fisheries, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
  • 3 College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
  • 4 College of Fisheries, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China; Hubei Provincial Engineering Laboratory for Pond Aquaculture, Hubei, China. Electronic address: gaojian@mail.hzau.edu.cn.
  • 5 College of Fisheries, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China; Hubei Provincial Engineering Laboratory for Pond Aquaculture, Hubei, China. Electronic address: caoxiaojuan@mail.hzau.edu.cn.
PMID: 37271265 DOI: 10.1016/j.ijbiomac.2023.125171
Abstract

Unreduced gametes, that are important for species evolution and agricultural development, are generally believed to be formed by meiotic defects. However, we found that male diploid loach (Misgurnus anguillicaudatus) could produce not only haploid sperms, but also unreduced sperms, after cyclin-dependent kinase 1 gene (CDK1, one of the most important kinases in regulating cell mitosis) deletion. Observations on synaptonemal complexes of spermatocyte in prophase of meiosis and spermatogonia suggested that the number of chromosomes in some spermatogonia of CDK1-/- loach doubled, leading to unreduced diploid sperm production. Then, transcriptome analysis revealed aberrant expressions of some cell cycle-related genes (such as ppp1c and gadd45) in spermatogonia of CDK1-/- loach relative to wild-type loach. An in vitro and in vivo experiment further validated that CDK1 deletion in diploid loach resulted in mitotic defects, leading to unreduced diploid sperm formation. In addition, we found that CDK1-/- zebrafish could also produce unreduced diploid sperms. This study provides important information on revealing the molecular mechanisms behind unreduced gamete formation through mitotic defects, and lays the foundation for a novel strategy for fish polyploidy creation by using CDK1 mutants to produce unreduced sperms, which can then be used to obtain polyploidy, proposed to benefit aquaculture.

Keywords

Cdk1 deletion; Mitotic defects; Unreduced sperms.

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