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  3. Generation of Human Blastoids from Naive Pluripotent Stem Cells

Generation of Human Blastoids from Naive Pluripotent Stem Cells

  • Methods Mol Biol. 2023 Jun 24. doi: 10.1007/7651_2023_485.
Yulei Wei 1 2,  Kun Liu 3 4,  Carlos A Pinzon-Arteaga 1,  Deirdre Logsdon 3,  Leqian Yu 1 5 6,  Ye Yuan 3,  Jun Wu 7 8 9
Affiliations

Affiliations

  • 1 Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
  • 2 State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
  • 3 Colorado Center for Reproductive Medicine, Lone Tree, CO, USA.
  • 4 Paul M. Rady Department of Mechanical Engineering, University of Colorado, Boulder, CO, USA.
  • 5 State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
  • 6 Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China.
  • 7 Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA. Jun2.Wu@UTSouthwestern.edu.
  • 8 Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA. Jun2.Wu@UTSouthwestern.edu.
  • 9 Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA. Jun2.Wu@UTSouthwestern.edu.
PMID: 37351840 DOI: 10.1007/7651_2023_485
Abstract

Under certain culture conditions, naive human pluripotent stem cells can generate human blastocyst-like structures (called human blastoids). Human blastoids serve as an accessible model for human blastocysts and are amenable for large-scale production. Here, we describe a detailed step-by-step protocol for the robust and high-efficient generation of human blastoids from naive human pluripotent stem cells.

Keywords

Human blastoids; Human extraembryonic cell types; Human implantation model; Hypoblast cells; Integrated models of human embryos; Naive human pluripotent stem cells; The generation of blastocyst-like structures; Trophoblast cells.

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