Ferroptosis contributes to ethanol-induced hepatic cell death via labile iron accumulation and GPx4 inactivation

Alcohol abuse is a significant cause of global morbidity and mortality, with alcoholic liver disease (ALD) being a common consequence. The pathogenesis of ALD involves various cellular processes, including oxidative stress, inflammation, and hepatic cell death. Recently, Ferroptosis, an iron-dependent form of programmed cell death, has emerged as a potential mechanism in many diseases. However, the specific involvement and regulatory mechanisms of Ferroptosis in ALD remain poorly understood. Here we aimed to investigate the presence and mechanism of alcohol-induced Ferroptosis and the involvement of miRNAs in regulating Ferroptosis sensitivity. Our findings revealed that long-term ethanol feeding induced Ferroptosis in male mice, as evidenced by increased expression of ferroptosis-related genes, lipid peroxidation, and labile iron accumulation in the liver. Furthermore, we identified dysregulation of the methionine cycle and transsulfuration pathway, leading to severe glutathione (GSH) exhaustion and indirect deactivation of Glutathione Peroxidase 4 (GPx4), a critical Enzyme in preventing Ferroptosis. Additionally, we identified miR-214 as a Ferroptosis regulator in ALD, enhancing hepatocyte Ferroptosis by transcriptionally activating the expression of ferroptosis-driver genes. Our study provides novel insights into the involvement and regulatory mechanisms of Ferroptosis in ALD, highlighting the potential therapeutic implications of targeting Ferroptosis and miRNAs in ALD management.