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  3. Evaluating anti-viral effect of Ivermectin on porcine epidemic diarrhea virus and analyzing the related genes and signaling pathway by RNA-seq in vitro

Evaluating anti-viral effect of Ivermectin on porcine epidemic diarrhea virus and analyzing the related genes and signaling pathway by RNA-seq in vitro

  • Virology. 2023 Sep 4;587:109877. doi: 10.1016/j.virol.2023.109877.
Yue Wang 1,  Panpan Qin 2,  Chenxu Zhao 3,  Yaqin Li 4,  Shuai Li 5,  Fangfang Fan 6,  Dongliang Li 7,  Huimin Huang 8,  Hong Duan 9,  Xia Yang 10,  Wenjuan Du 11,  Yongtao Li 12
Affiliations

Affiliations

  • 1 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China. Electronic address: yuewang0124@163.com.
  • 2 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China. Electronic address: 18238957691@163.com.
  • 3 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China. Electronic address: zhaochenxu0818@163.com.
  • 4 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China. Electronic address: 2916329547@qq.com.
  • 5 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China. Electronic address: 1513449376@qq.com.
  • 6 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China. Electronic address: 1060219965@qq.com.
  • 7 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Henan Agricultural University, Zhengzhou, 450046, China. Electronic address: 497222038@sina.com.
  • 8 College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, 6 Long-zi-hu Street, Zhengzhou, 450046, China. Electronic address: 18736090770@163.com.
  • 9 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Henan Agricultural University, Zhengzhou, 450046, China. Electronic address: duanhong0924@126.com.
  • 10 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Henan Agricultural University, Zhengzhou, 450046, China. Electronic address: yangxia66@163.com.
  • 11 Faculty of Veterinary Medicine, Utrecht University, Utrecht, 3584, CL, the Netherlands. Electronic address: w.du@uu.nl.
  • 12 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Henan Agricultural University, Zhengzhou, 450046, China; Faculty of Veterinary Medicine, Utrecht University, Utrecht, 3584, CL, the Netherlands. Electronic address: yongtaole@126.com.
PMID: 37688922 DOI: 10.1016/j.virol.2023.109877
Abstract

Porcine epidemic diarrhea virus (PEDV) has catastrophic impacts on the global pig industry. However, there remains no effective drugs for PEDV Infection. Ivermectin is an FDA-approved anthelmintic drug used to treat worm infections. In this study, we reported the broad-spectrum Antiviral activity of Ivermectin in vitro. Ivermectin can inhibit PEDV infections of different genotypes. Avermectin derivatives can also inhibit PEDV infections. A time of addition assay showed that Ivermectin exhibited potent anti-PEDV activity when added simultaneously with or post virus Infection. Furthermore, Ivermectin significantly inhibited the late stage of viral Infection by affecting viral release. RNA Sequencing indicates Ivermectin induces cell cycle arrest, which may be related to its ability to inhibit viral release. Interestingly, when combined with Niclosamide, Ivermectin demonstrated an enhanced anti-PEDV effect. These findings highlight Ivermectin as a novel Antiviral agent with potential for the development of drugs against PEDV Infection.

Keywords

Antiviral; Cell cycle arrest; Ivermectin; PEDV; Viral release.

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