2. Academic Validation
  3. Analysis of miRNA expression profiles in exosomes of SMB-S15 cells treated with resveratrol

Analysis of miRNA expression profiles in exosomes of SMB-S15 cells treated with resveratrol

  • Arch Virol. 2023 Oct 8;168(11):270. doi: 10.1007/s00705-023-05884-6.
Qiang Shi 1,  Lina Zhang 2,  Xiemusiye Wulayin 2,  Rundong Cao 2 3,  Mingyuan Jiao 1,  Jing Wang 2,  Jun Han 2 3,  Xiao-Ping Dong 4,  Chen Gao 5 6
Affiliations

Affiliations

  • 1 Tongzhou Maternal and Child Health Hospital of Beijing, Beijing, 101100, China.
  • 2 State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases (Zhejiang University), Chinese Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, Chang-Bai Rd 155, Beijing, 102206, People's Republic of China.
  • 3 National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases Center, Chang-Bai Rd 155, Beijing, 102206, China.
  • 4 State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases (Zhejiang University), Chinese Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, Chang-Bai Rd 155, Beijing, 102206, People's Republic of China. dongxiaoping@ivdc.chinacdc.cn.
  • 5 State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases (Zhejiang University), Chinese Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, Chang-Bai Rd 155, Beijing, 102206, People's Republic of China. gaochen@ivdc.chinacdc.cn.
  • 6 National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases Center, Chang-Bai Rd 155, Beijing, 102206, China. gaochen@ivdc.chinacdc.cn.
PMID: 37805966 DOI: 10.1007/s00705-023-05884-6
Abstract

Exosomes are double-layered vesicle bodies secreted by cells, in which MicroRNAs (miRNAs) play an important role. In a previous study, we found that treatment of the prion-infected cell line SMB-S15 with resveratrol can effectively inhibit the propagation of PrPSc in vitro and eliminate its infectivity in vivo. In this study, the global expression profiles of miRNAs in extracellular exosomes during resveratrol clearance of PrPSc in SMB-S15 cells were analyzed. Extracted exosomal miRNAs from the prion-infected cell line SMB-S15 (S15) and its normal partner cell line SMB-PS (PS) as well as SMB-S15 cells exposed to resveratrol for 4 days (RES4) and 8 days (RES8) were subjected into deep Sequencing. Similarities and differences in the levels of differentially expressed miRNAs as well as the signaling pathways that are potentially involved were comparatively analyzed. The possible influences on the expression of genes affected by changes in exosomal miRNAs in the context of the prion pathway were further analyzed. These alterations in exosomal miRNA levels may help us to understand the functional transmission of intercellular messages and the pathogenesis of prion biology and prion disease.

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