2. Academic Validation
  3. METTL14-mediated m6A mRNA modification of G6PD promotes lung adenocarcinoma

METTL14-mediated m6A mRNA modification of G6PD promotes lung adenocarcinoma

  • Cell Death Discov. 2024 Aug 13;10(1):361. doi: 10.1038/s41420-024-02133-w.
Weidong Wu # 1 2 3 4,  Mengling Li # 5 6,  Yingxiao Wu # 5 6,  Qiongying Wei 5 6,  Nanding Yu 7 8
Affiliations

Affiliations

  • 1 Department of Thoracic Surgery, Fujian Medical University Union Hospital, Fuzhou, 350001, Fujian, China.
  • 2 Fujian Key Laboratory of Cardio-Thoracic Surgery, Fujian Medical University, Fuzhou, 350122, Fujian, China.
  • 3 National Key Clinical Specialty of Thoracic Surgery, Fuzhou, 350001, Fujian, China.
  • 4 Clinical Research Center for Thoracic Tumors of Fujian Province, Fuzhou, 350001, Fujian, China.
  • 5 Department of Pulmonary and Critical Care Medicine, Fujian Medical University Union Hospital, Fuzhou, 350001, Fujian, China.
  • 6 Department of Geriatric Medicine, Fujian Medical University Union Hospital, Fuzhou, 350001, Fujian, China.
  • 7 Department of Pulmonary and Critical Care Medicine, Fujian Medical University Union Hospital, Fuzhou, 350001, Fujian, China. yunanding@fjmu.edu.cn.
  • 8 Department of Geriatric Medicine, Fujian Medical University Union Hospital, Fuzhou, 350001, Fujian, China. yunanding@fjmu.edu.cn.
  • # Contributed equally.
PMID: 39138186 DOI: 10.1038/s41420-024-02133-w
Abstract

METTL14 functions as an RNA methyltransferase involved in m6A modification, influencing mRNA biogenesis, decay, and translation processes. However, the specific mechanism by which METTL14 regulates glucose-6-phosphate dehydrogenase (G6PD) to promote the progression of lung adenocarcinoma (LUAD) is not well understood. Quantitative measurement and immunohistochemistry (IHC) analysis have demonstrated higher levels of m6A in LUAD tissues compared to adjacent normal tissues. Additionally, the expression of METTL14 was significantly increased in LUAD tissues. In LUAD cell lines, both METTL14 and m6A levels were elevated compared to normal human lung epithelial cells. Knockdown of METTL14 markedly reduced LUAD cell proliferation, migration, and invasion. Conversely, overexpression of METTL14, but not the mutant form, significantly enhanced these cellular processes in LUAD. In vivo studies using nude mice with subcutaneously transplanted LUAD cells demonstrated that stable METTL14 knockdown led to notably reduced tumor volume and weight, along with fewer Ki67-positive cells and lung metastatic sites. Importantly, METTL14 knockdown reduced glycolytic activity in LUAD cells. Through a combination of RNA Sequencing and MeRIP-sequencing, we identified numerous altered genes and confirmed that IGF2BP2 enhances G6PD mRNA stability after METTL14-mediated m6A modification, thereby promoting tumor growth and metastasis. Moreover, LUAD patients with higher levels of G6PD had poorer overall survival (OS). In conclusion, our study indicates that METTL14 upregulates G6PD expression post-transcriptionally through an m6A-IGF2BP2-dependent mechanism, thereby stabilizing G6PD mRNA. These findings propose potential diagnostic biomarkers and effective targets for anti-metabolism therapy in LUAD.

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