Actinomycin D (Synonyms: 放线菌素 D; Dactinomycin; Actinomycin IV)

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Actinomycin D (Dactinomycin) 抑制 DNA 修复，IC₅₀ 为 0.42 μM。Actinomycin D 是一种自噬 (autophagy) 激活剂。

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Actinomycin D Chemical Structure

CAS No. : 50-76-0

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MCE 顾客使用本产品发表的 492 篇科研文献

RT-PCR

: Actinomycin D purchased from MCE. Usage Cited in: Cell Death Dis. 2019 May 21;10(6):395. [Abstract]; Actinomycin D ( Act D; 5μg/ml; 0.5, 1, 2, 4 h) increases the expression of Mcl-1 protein.

: Actinomycin D purchased from MCE. Usage Cited in: Cancer Lett. 2017 Oct 28;407:45-56. [Abstract]; UM-SCC6 and UM-SCC6H cells are treated with Actinomycin D (ActD 5 μg/mL) for the indicated times. The IGF2 mRNA level is measured by RT-PCR.

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生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

Actinomycin D (Dactinomycin) inhibits DNA repair with an IC₅₀ of 0.42 μM. Actinomycin D is an autophagy activator^[1]^[2]^[3].

IC₅₀ & Target

IC50: 0.42 μM (DNA repair)^[1]

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
5637	IC₅₀	0.003 μM Compound: AMD	Antiproliferative activity against human 5637 cell line by MTT assay Antiproliferative activity against human 5637 cell line by MTT assay	[PMID: 16913700]
A2780	IC₅₀	1 ng/mL Compound: actinomycin D	Cytotoxicity against human A2780 cells by clonogenic assay Cytotoxicity against human A2780 cells by clonogenic assay	[PMID: 16499334]
A549	EC₅₀	0.201 nM Compound: D	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by alamar blue assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by alamar blue assay	[PMID: 27736087]
A549	IC₅₀	0.8 x 10^-2 μM Compound: C1	Cytotoxicity against human A549 cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay	[PMID: 28923386]
A549	IC₅₀	3.8 μM Compound: 1	Cytotoxicity against human A549 cells assessed as growth inhibition incubated for 68 hrs by MTT assay Cytotoxicity against human A549 cells assessed as growth inhibition incubated for 68 hrs by MTT assay	[PMID: 34270246]
A549	IC₅₀	7.2 nM Compound: C1	Cytotoxicity against human A549 cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay	[PMID: 28923386]
A549	IC₅₀	7.2 nM Compound: C1	Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 22705001]
ACHN	IC₅₀	0.005 μM Compound: AMD	Antiproliferative activity against human ACHN cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human ACHN cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]
ACHN	IC₅₀	0.005 μM Compound: AMD	Antiproliferative activity against human ACHN cell line by MTT assay Antiproliferative activity against human ACHN cell line by MTT assay	[PMID: 16913700]
BEAS-2B	IC₅₀	0.7 μM Compound: 1	Cytotoxicity against human BEAS-2B cells assessed as growth inhibition incubated for 68 hrs by MTT assay Cytotoxicity against human BEAS-2B cells assessed as growth inhibition incubated for 68 hrs by MTT assay	[PMID: 34270246]
C8166	IC₅₀	0.0008 μM Compound: AMD	Antiproliferative activity against human C8166 cell line by MTT assay Antiproliferative activity against human C8166 cell line by MTT assay	[PMID: 16913700]
Ca-Ski	ED₅₀	1.9 ng/mL Compound: Actinomycin D	Cytotoxicity against human CaSki cells after 6 days by MTT assay Cytotoxicity against human CaSki cells after 6 days by MTT assay	[PMID: 9917310]
Ca-Ski	ED₅₀	1.9 ng/mL Compound: actinomycin D	Cytotoxicity against human CaSKi cells after 6 days by MTT assay Cytotoxicity against human CaSKi cells after 6 days by MTT assay	[PMID: 10785428]
Ca-Ski	ED₅₀	1.9 ng/mL Compound: actinomycin D	Cytotoxicity against human CaSki cells after 6 days by MTT assay Cytotoxicity against human CaSki cells after 6 days by MTT assay	[PMID: 10425143]
Ca-Ski	ED₅₀	1.9 ng/mL Compound: actinomycin D	Cytotoxicity against human CaSKi cells by MTT assay Cytotoxicity against human CaSKi cells by MTT assay	[PMID: 11908984]
CCRF-CEM	IC₅₀	0.001 μM Compound: AMD	Antiproliferative activity against human CCRF-CEM cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human CCRF-CEM cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]
CCRF-CEM	IC₅₀	0.001 μM Compound: AMD	Antiproliferative activity against human CCRF-CEM cell line by MTT assay Antiproliferative activity against human CCRF-CEM cell line by MTT assay	[PMID: 16913700]
CCRF-CEM	IC₅₀	1.6 nM Compound: actinomycin D	Cytotoxicity against human CCRF-CEM cells after 72 hrs Cytotoxicity against human CCRF-CEM cells after 72 hrs	[PMID: 18329887]
CCRF-SB	IC₅₀	0.002 μM Compound: AMD	Antiproliferative activity against human CCRF-SB cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human CCRF-SB cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]
CCRF-SB	IC₅₀	0.002 μM Compound: AMD	Antiproliferative activity against human CCRF-SB cell line by MTT assay Antiproliferative activity against human CCRF-SB cell line by MTT assay	[PMID: 16913700]
G-361	IC₅₀	0.001 μM Compound: AMD	Antiproliferative activity against human G361 cell line by MTT assay Antiproliferative activity against human G361 cell line by MTT assay	[PMID: 16913700]
G-361	IC₅₀	0.006 μM Compound: AMD	Antiproliferative activity against human G-361 cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human G-361 cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]
HEK293	IC₅₀	9 μM Compound: 1	Inhibition of human DDR2 expressed in HEK293 cells assessed as reduction in collagen-1-induced DDR2 tyrosine phosphorylation pre-incubated for 30 mins by immunoblotting method Inhibition of human DDR2 expressed in HEK293 cells assessed as reduction in collagen-1-induced DDR2 tyrosine phosphorylation pre-incubated for 30 mins by immunoblotting method	[PMID: 25569119]
HeLa	IC₅₀	0.001 μM Compound: actinomycin D	Cytotoxicity against human HeLa cells in log-phase after 48 hrs by MTT assay Cytotoxicity against human HeLa cells in log-phase after 48 hrs by MTT assay	[PMID: 16309323]
HeLa	IC₅₀	0.008 μM Compound: AMD	Antiproliferative activity against human HeLa cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human HeLa cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]
HeLa	IC₅₀	0.01 μg/mL Compound: Actinomycin D	Cytotoxicity against human HeLa cells after 3 days by methylene blue assay Cytotoxicity against human HeLa cells after 3 days by methylene blue assay	[PMID: 9644081]
HeLa	IC₅₀	0.011 μM Compound: actinomycin D	Cytotoxicity against human HeLa cells in lag-phase after 48 hrs by MTT assay Cytotoxicity against human HeLa cells in lag-phase after 48 hrs by MTT assay	[PMID: 16309323]
HeLa	IC₅₀	0.04 μM Compound: AMD	Antiproliferative activity against human HeLa cells by MTT assay Antiproliferative activity against human HeLa cells by MTT assay	[PMID: 16913700]
HeLa	IC₅₀	0.12 μM Compound: 1	Cytotoxicity against human HeLa cells assessed as growth inhibition incubated for 68 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as growth inhibition incubated for 68 hrs by MTT assay	[PMID: 34270246]
HeLa	IC₅₀	0.4 nM Compound: Actinomycin D	Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay	[PMID: 18461997]
HeLa	IC₅₀	1.1 nM Compound: C1	Cytotoxicity against human HeLa cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay	[PMID: 28923386]
HeLa	IC₅₀	1.1 nM Compound: C1	Cytotoxicity against human HeLa cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 22705001]
HeLa	IC₅₀	1.1 nM Compound: Actinomycin D	Cytotoxicity against human HeLa cells in lag phase of growth after 48 hrs Cytotoxicity against human HeLa cells in lag phase of growth after 48 hrs	[PMID: 24709561]
HeLa	IC₅₀	1.6 nM Compound: Actinomycin D	Cytotoxicity against human HeLa cells in log phase of growth after 48 hrs Cytotoxicity against human HeLa cells in log phase of growth after 48 hrs	[PMID: 24709561]
HeLa	IC₅₀	262.4 nM Compound: ActD	Inhibition of transcriptional activity in human HeLa cells assessed as [3H]uridine utilization after 2 hrs by scintillation counting Inhibition of transcriptional activity in human HeLa cells assessed as [3H]uridine utilization after 2 hrs by scintillation counting	[PMID: 20118940]
HeLa	IC₅₀	3.2 nM Compound: C1	Cytotoxicity against human HeLa cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay	[PMID: 28923386]
HeLa	IC₅₀	519.7 nM Compound: ActD	Inhibition of protein synthesis in human HeLa cells assessed as [35S]cysteine/methionine utilization after 2 hrs by scintillation spectroscopy Inhibition of protein synthesis in human HeLa cells assessed as [35S]cysteine/methionine utilization after 2 hrs by scintillation spectroscopy	[PMID: 20118940]
Hep 3B2	IC₅₀	0.01 μg/mL Compound: Actinomycin D	Cytotoxicity against human Hep3B cells after 3 days by methylene blue assay Cytotoxicity against human Hep3B cells after 3 days by methylene blue assay	[PMID: 9644081]
HEp-2	IC₅₀	0.004 μM Compound: AMD	Antiproliferative activity against human Hep2 cell line by MTT assay Antiproliferative activity against human Hep2 cell line by MTT assay	[PMID: 16913700]
HEp-2	IC₅₀	0.006 μM Compound: actinomycin D	Cytotoxicity against human Hep2 cells in log-phase after 48 hrs by MTT assay Cytotoxicity against human Hep2 cells in log-phase after 48 hrs by MTT assay	[PMID: 16309323]
HEp-2	IC₅₀	0.191 μM Compound: actinomycin D	Cytotoxicity against human Hep2 cells in lag-phase after 48 hrs by MTT assay Cytotoxicity against human Hep2 cells in lag-phase after 48 hrs by MTT assay	[PMID: 16309323]
HEp-2	IC₅₀	1.7 x 10^-1 μM Compound: Actinomycin D	Cytotoxicity against human Hep2 cells in lag phase of growth after 48 hrs Cytotoxicity against human Hep2 cells in lag phase of growth after 48 hrs	[PMID: 24709561]
HEp-2	IC₅₀	6.2 nM Compound: Actinomycin D	Cytotoxicity against human Hep2 cells in log phase of growth after 48 hrs Cytotoxicity against human Hep2 cells in log phase of growth after 48 hrs	[PMID: 24709561]
HepG2	IC₅₀	0.01 μM Compound: 1	Cytotoxicity against human HepG2 cells assessed as growth inhibition incubated for 68 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as growth inhibition incubated for 68 hrs by MTT assay	[PMID: 34270246]
HepG2	GI₅₀	1 μM Compound: actinomycin D	Cytotoxicity against human HepG2 cells after 48 hrs by sulforhodamine assay Cytotoxicity against human HepG2 cells after 48 hrs by sulforhodamine assay	[PMID: 10757717]
HL-60	IC₅₀	0.02 μM Compound: 1	Cytotoxicity against human HL-60 cells assessed as growth inhibition incubated for 68 hrs by MTT assay Cytotoxicity against human HL-60 cells assessed as growth inhibition incubated for 68 hrs by MTT assay	[PMID: 34270246]
HT-29	IC₅₀	0.006 μM Compound: AMD	Antiproliferative activity against human HT-29 cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human HT-29 cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]
HT-29	IC₅₀	0.006 μM Compound: AMD	Antiproliferative activity against human HT29 cell line by MTT assay Antiproliferative activity against human HT29 cell line by MTT assay	[PMID: 16913700]
HT-29	IC₅₀	2.9 nM Compound: Actinomycin D	Antiproliferative activity against human HT29 cells after 48 hrs by MTT assay Antiproliferative activity against human HT29 cells after 48 hrs by MTT assay	[PMID: 18461997]
HT-29	IC₅₀	223 nM Compound: Actinomycin D	Antiproliferative activity against human HT-29 cells after 96 hrs by MTT assay Antiproliferative activity against human HT-29 cells after 96 hrs by MTT assay	[PMID: 2778449]
HT-3	ED₅₀	5.6 x 10^-4 μg/mL Compound: Actinomycin D	Cytotoxicity against human HT-3 cells after 6 days by MTT assay Cytotoxicity against human HT-3 cells after 6 days by MTT assay	[PMID: 9917310]
HT-3	ED₅₀	5.6 x 10^-4 μg/mL Compound: actinomycin D	Cytotoxicity against human HT3 cells after 6 days by MTT assay Cytotoxicity against human HT3 cells after 6 days by MTT assay	[PMID: 10785428]
HT-3	ED₅₀	5.6 x 10^-4 μg/mL Compound: actinomycin D	Cytotoxicity against human HT-3 cells after 6 days by MTT assay Cytotoxicity against human HT-3 cells after 6 days by MTT assay	[PMID: 10425143]
Jurkat	IC₅₀	0.0045 μM Compound: Actinomycin D	Inhibitory concentration was evaluated on human Jurkat T-cells after their exposure for 48 hours Inhibitory concentration was evaluated on human Jurkat T-cells after their exposure for 48 hours	[PMID: 11052798]
KB	IC₅₀	0.001 μM Compound: AMD	Antiproliferative activity against human KB cell line by MTT assay Antiproliferative activity against human KB cell line by MTT assay	[PMID: 16913700]
KB	IC₅₀	0.004 μM Compound: Actinomycin D	Concentration required to reduce proliferation of KB human nasopharyngeal carcinoma (KBWT) cell line by 50% as determined by the MTT method Concentration required to reduce proliferation of KB human nasopharyngeal carcinoma (KBWT) cell line by 50% as determined by the MTT method	[PMID: 14761187]
KB	IC₅₀	0.005 μM Compound: AMD	Compound concentration required to reduce the cell proliferation of Wild type and Drug- resistant human nasopharyngeal carcinoma KB cells by 50% Compound concentration required to reduce the cell proliferation of Wild type and Drug- resistant human nasopharyngeal carcinoma KB cells by 50%	[PMID: 12431048]
KB	IC₅₀	0.01 μg/mL Compound: Actinomycin D	Cytotoxicity against human KB cells after 72 hrs by methylene blue assay Cytotoxicity against human KB cells after 72 hrs by methylene blue assay	[PMID: 12502326]
KB	IC₅₀	0.01 μg/mL Compound: Actinomycin D	Cytotoxicity against human KB cells after 72 hrs by methylene blue assay Cytotoxicity against human KB cells after 72 hrs by methylene blue assay	[PMID: 11473432]
KB	IC₅₀	0.05 μM Compound: Actinomycin D	Concentration required to reduce proliferation of KB subclones passaged in the presence of vincristine 0.02 uM (KB7D) cell line by 50% as determined by the MTT method Concentration required to reduce proliferation of KB subclones passaged in the presence of vincristine 0.02 uM (KB7D) cell line by 50% as determined by the MTT method	[PMID: 14761187]
KB	IC₅₀	0.2 μM Compound: Actinomycin D	Concentration required to reduce proliferation of KB subclones passaged in the presence of etoposide 7 uM (KB7D) cell line by 50% as determined by the MTT method Concentration required to reduce proliferation of KB subclones passaged in the presence of etoposide 7 uM (KB7D) cell line by 50% as determined by the MTT method	[PMID: 14761187]
KB	IC₅₀	0.2 μM Compound: Actinomycin D	Concentration required to reduce proliferation of KB subclones passaged in the presence of doxorubicin 0.09 uM (KBMDR) cell line by 50% as determined by the MTT method Concentration required to reduce proliferation of KB subclones passaged in the presence of doxorubicin 0.09 uM (KBMDR) cell line by 50% as determined by the MTT method	[PMID: 14761187]
L02	IC₅₀	0.5 μM Compound: 1	Cytotoxicity against human L02 cells assessed as growth inhibition incubated for 68 hrs by MTT assay Cytotoxicity against human L02 cells assessed as growth inhibition incubated for 68 hrs by MTT assay	[PMID: 34270246]
L1210	ED₅₀	0.009 μM Compound: 1	Inhibition of RNA synthesis in mouse L1210 cells assessed as [3H]-uridine incorporation preincubated for 3 hrs before [3H]-uridine addition measured after 1 hr by scintillation spectrometric analysis Inhibition of RNA synthesis in mouse L1210 cells assessed as [3H]-uridine incorporation preincubated for 3 hrs before [3H]-uridine addition measured after 1 hr by scintillation spectrometric analysis	[PMID: 330857]
L1210	ED₅₀	0.015 μM Compound: 1	Inhibition of RNA synthesis in mouse L1210 cells by [3H]-uridine incorporation assay Inhibition of RNA synthesis in mouse L1210 cells by [3H]-uridine incorporation assay	[PMID: 573799]
L1210	ED₅₀	0.4 μM Compound: 1	Inhibition of DNA synthesis in mouse L1210 cells assessed as [3H]-thymidine incorporation preincubated for 3 hrs before [3H]-thymidine addition measured after 1 hr by scintillation spectrometric analysis Inhibition of DNA synthesis in mouse L1210 cells assessed as [3H]-thymidine incorporation preincubated for 3 hrs before [3H]-thymidine addition measured after 1 hr by scintillation spectrometric analysis	[PMID: 330857]
L1210	ED₅₀	0.6 μM Compound: 1	Inhibition of DNA synthesis in mouse L1210 cells by [3H]-thymidine incorporation assay Inhibition of DNA synthesis in mouse L1210 cells by [3H]-thymidine incorporation assay	[PMID: 573799]
MCF7	IC₅₀	0.006 μM Compound: AMD	Antiproliferative activity against human MCF7 cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human MCF7 cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]
MCF7	IC₅₀	0.103 μM Compound: actinomycin D	Cytotoxicity against human MCF7 cells after 72 hrs by MTS reduction assay Cytotoxicity against human MCF7 cells after 72 hrs by MTS reduction assay	[PMID: 20704331]
MCF7	IC₅₀	0.2 μM Compound: 1	Cytotoxicity against human MCF7 cells assessed as growth inhibition incubated for 68 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as growth inhibition incubated for 68 hrs by MTT assay	[PMID: 34270246]
MCF7	GI₅₀	0.5 μM Compound: actinomycin D	Cytotoxicity against human MCF7 cells after 48 hrs by sulforhodamine assay Cytotoxicity against human MCF7 cells after 48 hrs by sulforhodamine assay	[PMID: 10757717]
MCF7	IC₅₀	2.4 x 10^-2 μM Compound: C1	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay	[PMID: 28923386]
MCF7	IC₅₀	2.4 x 10^-2 μM Compound: C1	Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 22705001]
MCF7	IC₅₀	4.8 x 10^-2 μM Compound: C1	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay	[PMID: 28923386]
MOLT-3	IC₅₀	< 1 μM Compound: Actinomycin D	Cytotoxicity against MOLT3 Leukemia cells by MTS assay Cytotoxicity against MOLT3 Leukemia cells by MTS assay	[PMID: 16420056]
MOLT-3	IC₅₀	≤ 1 μM Compound: Actinomycin D	Cytotoxicity against human MOLT3 cells after 20 hrs by MTS assay Cytotoxicity against human MOLT3 cells after 20 hrs by MTS assay	[PMID: 21093966]
MOLT-4	IC₅₀	0.001 μM Compound: AMD	Antiproliferative activity against human MOLT4 cell line by MTT assay Antiproliferative activity against human MOLT4 cell line by MTT assay	[PMID: 16913700]
MT4	IC₅₀	0.0009 μM Compound: AMD	Antiproliferative activity against human MT4 cell line by MTT assay Antiproliferative activity against human MT4 cell line by MTT assay	[PMID: 16913700]
MT4	IC₅₀	0.001 μM Compound: AMD	Antiproliferative activity against human MT-4 cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human MT-4 cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]
NCI-H460	IC₅₀	0.008 μM Compound: actinomycin D	Cytotoxicity against human NCI-H460 cells after 72 hrs by MTS reduction assay Cytotoxicity against human NCI-H460 cells after 72 hrs by MTS reduction assay	[PMID: 20704331]
P388	ED₅₀	0.046 μM Compound: AMD	Concentration required for 50% inhibition of incorporation of labeled uridine by P388 cells for the synthesis of RNA (in vitro) Concentration required for 50% inhibition of incorporation of labeled uridine by P388 cells for the synthesis of RNA (in vitro)	[PMID: 6169834]
P388	IC₅₀	0.2 nM Compound: Actinomycin D	Antiproliferative activity against mouse P388 cells after 48 hrs by MTT assay Antiproliferative activity against mouse P388 cells after 48 hrs by MTT assay	[PMID: 2778449]
P388	ED₅₀	0.996 μM Compound: AMD	Concentration required for 50% inhibition of incorporation of labeled thymidine by p388 cells for synthesis of DNA (in vitro) Concentration required for 50% inhibition of incorporation of labeled thymidine by p388 cells for synthesis of DNA (in vitro)	[PMID: 6169834]
PC-3	EC₅₀	0.276 nM Compound: D	Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 48 hrs by alamar blue assay Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 48 hrs by alamar blue assay	[PMID: 27736087]
PLC-PRF-5	ED₅₀	1.4 ng/mL Compound: Actinomycin D	Cytotoxicity against human PLC/PRF/5 cells after 6 days by MTT assay Cytotoxicity against human PLC/PRF/5 cells after 6 days by MTT assay	[PMID: 9917310]
PLC-PRF-5	ED₅₀	1.4 ng/mL Compound: actinomycin D	Cytotoxicity against human PLC/PRF/5 cells after 6 days by MTT assay Cytotoxicity against human PLC/PRF/5 cells after 6 days by MTT assay	[PMID: 10785428]
PLC-PRF-5	ED₅₀	1.4 ng/mL Compound: actinomycin D	Cytotoxicity against human PLC/PRF/5 cells after 6 days by MTT assay Cytotoxicity against human PLC/PRF/5 cells after 6 days by MTT assay	[PMID: 10425143]
RKO	IC₅₀	0.03 μM Compound: 1	Cytotoxicity against human RKO cells assessed as growth inhibition incubated for 68 hrs by MTT assay Cytotoxicity against human RKO cells assessed as growth inhibition incubated for 68 hrs by MTT assay	[PMID: 34270246]
SF-268	IC₅₀	0.016 μM Compound: actinomycin D	Cytotoxicity against human SF268 cells after 72 hrs by MTS reduction assay Cytotoxicity against human SF268 cells after 72 hrs by MTS reduction assay	[PMID: 20704331]
SiHa	ED₅₀	8.1 x 10^-4 μg/mL Compound: Actinomycin D	Cytotoxicity against human SiHa cells after 6 days by MTT assay Cytotoxicity against human SiHa cells after 6 days by MTT assay	[PMID: 9917310]
SiHa	ED₅₀	8.1 x 10^-4 μg/mL Compound: actinomycin D	Cytotoxicity against human SiHa cells after 6 days by MTT assay Cytotoxicity against human SiHa cells after 6 days by MTT assay	[PMID: 10785428]
SiHa	ED₅₀	8.1 x 10^-4 μg/mL Compound: actinomycin D	Cytotoxicity against human SiHa cells after 6 days by MTT assay Cytotoxicity against human SiHa cells after 6 days by MTT assay	[PMID: 10425143]
SiHa	ED₅₀	8.1 x 10^-4 μg/mL Compound: actinomycin D	Cytotoxicity against human SiHa cells by MTT assay Cytotoxicity against human SiHa cells by MTT assay	[PMID: 11908984]
SK-MEL-28	IC₅₀	0.002 μM Compound: AMD	Antiproliferative activity against human SKMEL-28 cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human SKMEL-28 cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]
T-24	ED₅₀	1.5 ng/mL Compound: Actinomycin D	Cytotoxicity against human T24 cells after 6 days by MTT assay Cytotoxicity against human T24 cells after 6 days by MTT assay	[PMID: 9917310]
T-24	ED₅₀	1.5 ng/mL Compound: actinomycin D	Cytotoxicity against human T24 cells after 6 days by MTT assay Cytotoxicity against human T24 cells after 6 days by MTT assay	[PMID: 10785428]
T-24	ED₅₀	1.5 ng/mL Compound: actinomycin D	Cytotoxicity against human T24 cells after 6 days by MTT assay Cytotoxicity against human T24 cells after 6 days by MTT assay	[PMID: 10425143]
U2OS	IC₅₀	3.2 nM Compound: Actinomycin D	Antiproliferative activity against human U2OS cells after 48 hrs by MTT assay Antiproliferative activity against human U2OS cells after 48 hrs by MTT assay	[PMID: 18461997]
U-937	IC₅₀	< 1 μM Compound: Actinomycin D	Cytotoxicity against U937 Lymphoma cells by MTS assay Cytotoxicity against U937 Lymphoma cells by MTS assay	[PMID: 16420056]
Vero	IC₅₀	0.001 μM Compound: actinomycin D	Cytotoxicity against african green monkey Vero cells in log-phase after 48 hrs by MTT assay Cytotoxicity against african green monkey Vero cells in log-phase after 48 hrs by MTT assay	[PMID: 16309323]
Vero	IC₅₀	0.071 μM Compound: actinomycin D	Cytotoxicity against african green monkey Vero cells in lag-phase after 48 hrs by MTT assay Cytotoxicity against african green monkey Vero cells in lag-phase after 48 hrs by MTT assay	[PMID: 16309323]
Vero	IC₅₀	1 x 10^-2 μM Compound: Actinomycin D	Cytotoxicity against African green monkey Vero cells in log phase of growth after 48 hrs Cytotoxicity against African green monkey Vero cells in log phase of growth after 48 hrs	[PMID: 24709561]
Vero	IC₅₀	1.1 x 10^-2 μM Compound: C1	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay	[PMID: 28923386]
Vero	IC₅₀	11 μM Compound: Actinomycin D	Cytotoxicity against african green monkey Vero cells after 20 hrs by MTS assay Cytotoxicity against african green monkey Vero cells after 20 hrs by MTS assay	[PMID: 21093966]
Vero	IC₅₀	11 μM Compound: Actinomycin D	Cytotoxicity against normal vero kidney cells by MTS assay Cytotoxicity against normal vero kidney cells by MTS assay	[PMID: 16420056]
Vero	IC₅₀	7.1 x 10^-2 μM Compound: C1	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay	[PMID: 28923386]
Vero	IC₅₀	7.1 x 10^-2 μM Compound: C1	Cytotoxicity against african green monkey Vero cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against african green monkey Vero cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 22705001]
Vero	IC₅₀	7.2 x 10^-2 μM Compound: Actinomycin D	Cytotoxicity against African green monkey Vero cells in lag phase of growth after 48 hrs Cytotoxicity against African green monkey Vero cells in lag phase of growth after 48 hrs	[PMID: 24709561]
WIL2-NS	IC₅₀	0.003 μM Compound: AMD	Antiproliferative activity against human Wil2-NS cells by MTT assay Antiproliferative activity against human Wil2-NS cells by MTT assay	[PMID: 16913700]

体外研究
(In Vitro)

放线菌素 D (Dactinomycin) 是 DNA 转录和复制的抑制剂^[1]。Actinomycin D 在 80 nM 时通过抑制 BrdU 掺入显著减少血管平滑肌细胞 (SMC) 的增殖。使用流式细胞仪分析的 G1 期停滞进一步支持了这一点。Actinomycin D 非常有效，抑制浓度 IC₅₀ 为 0.4 nM，而致死剂量 LD50 为 260 microM。增殖细胞核抗原 (PCNA)、黏着斑激酶 (FAK) 和 Raf 的蛋白表达水平均被放线菌素 D 抑制。参与细胞周期停滞的细胞外信号调节激酶 (Erk) 被发现被放线菌素 D 增加^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

将含有 80 nM 和 80 μM Actinomycin D (Dactinomycin) 的普朗尼克凝胶局部涂抹在大鼠颈动脉外膜周围，新生内膜厚度显著减少 (分别为 45% 和 55%)^[2]。放线菌素 D 和 NSC 118218 组小鼠的寿命明显长于对照组，P 值分别为 <0.001 和 0.007。有趣的是，放线菌素 D 单药处理的总生存率优于 NSC 118218 (P=0.026)^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1255.42

Formula

C₆₂H₈₆N₁₂O₁₆

CAS 号

50-76-0

性状

固体

颜色

Orange to red

中文名称

放线菌素 D

结构分类

初始来源

微生物

Streptomyces

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

细胞实验：

DMSO 中的溶解度 : 100 mg/mL (79.65 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	0.7965 mL	3.9827 mL	7.9655 mL
5 mM	0.1593 mL	0.7965 mL	1.5931 mL
10 mM	0.0797 mL	0.3983 mL	0.7965 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (1.99 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (1.66 mM); 悬浊液; 超声助溶

此方案可获得 2.08 mg/mL的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.58%

选择批次：

Data Sheet (659 KB) SDS (644 KB)

COA (186 KB) LCMS (437 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Barret JM, et al. Evaluation of DNA repair inhibition by antitumor or antibiotic drugs using a chemiluminescence microplateassay. Carcinogenesis. 1997 Dec;18(12):2441-5. [Content Brief]

[2]. Wu CH, et al. The molecular mechanism of actinomycin D in preventing neointimal formation in rat carotid arteries after balloon injury. J Biomed Sci. 2005;12(3):503-12. [Content Brief]

[3]. Merkel O, et al. Actinomycin D induces p53-independent cell death and prolongs survival in high-risk chronic lymphocytic leukemia. Leukemia. 2012 Dec;26(12):2508-16. [Content Brief]

Kinase Assay ^[1]	Actinomycin D is co-incubated for 3 h at 30°C with a reaction mixture containing: 120 mg of a whole-cell extract of HeLa cells, 70 mM KCl, 0.4 mM each of dGTP, dCTP, dATP, and digoxygenylated-dUTP in reaction buffer containing 40mM Hepes-KOH (pH 7.6), 5 mM MgCl₂, 0.5 mM Dithiotreitol, 2 mM EGTA, 10 mM phosphocreatine, 50 mg/mL creatine phosphate, and 360 mg/mL of bovine serum albumin. During this reaction, DNA damage is recognized and the excised patches are replaced by neosynthesized DNA fragments. Throughout this DNA synthesis, digoxygenylated-dUMPs are incorporated. The DNA repair reaction is stopped by three washes^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[3]	Mice^[3] The original Eμ-TCL1a transgenic mice have been backcrossed to C57BL/6 mice for >9 generations.The C57BL/6 wild-type mice are engrafted with tumor cells from Eμ-TCL-1 transgenic mice. The percentage of CD5+/CD19+ cells in the peripheral blood is routinely checked in mice by taking blood from the tail vein and analyzing it via flow cytometry. When the percentage of tumor cells in the peripheral blood reached 40-60%, treatment is started. Actinomycin D (0.06 mg/kg by 10 days) is applied daily via i.p. injections. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Barret JM, et al. Evaluation of DNA repair inhibition by antitumor or antibiotic drugs using a chemiluminescence microplateassay. Carcinogenesis. 1997 Dec;18(12):2441-5. [Content Brief] [2]. Wu CH, et al. The molecular mechanism of actinomycin D in preventing neointimal formation in rat carotid arteries after balloon injury. J Biomed Sci. 2005;12(3):503-12. [Content Brief] [3]. Merkel O, et al. Actinomycin D induces p53-independent cell death and prolongs survival in high-risk chronic lymphocytic leukemia. Leukemia. 2012 Dec;26(12):2508-16. [Content Brief]

Actinomycin D 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.7965 mL	3.9827 mL	7.9655 mL	19.9137 mL
	5 mM	0.1593 mL	0.7965 mL	1.5931 mL	3.9827 mL
	10 mM	0.0797 mL	0.3983 mL	0.7965 mL	1.9914 mL
	15 mM	0.0531 mL	0.2655 mL	0.5310 mL	1.3276 mL
	20 mM	0.0398 mL	0.1991 mL	0.3983 mL	0.9957 mL
	25 mM	0.0319 mL	0.1593 mL	0.3186 mL	0.7965 mL
	30 mM	0.0266 mL	0.1328 mL	0.2655 mL	0.6638 mL
	40 mM	0.0199 mL	0.0996 mL	0.1991 mL	0.4978 mL
	50 mM	0.0159 mL	0.0797 mL	0.1593 mL	0.3983 mL
	60 mM	0.0133 mL	0.0664 mL	0.1328 mL	0.3319 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Actinomycin D50-76-0Dactinomycin Actinomycin IV放线菌素 DDNA/RNA SynthesisAutophagyBacterialApoptosisAntibioticDNArepairtranscriptionreplicationInhibitorinhibitorinhibit

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Actinomycin D (Synonyms: 放线菌素 D; Dactinomycin; Actinomycin IV)

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Actinomycin D (Synonyms: 放线菌素 D; Dactinomycin; Actinomycin IV)

Customer Review

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