2. Academic Validation
  3. Acrolein scavengers and detoxification: From high-throughput screening of flavonoids to mechanistic study of epigallocatechin gallate

Acrolein scavengers and detoxification: From high-throughput screening of flavonoids to mechanistic study of epigallocatechin gallate

  • J Hazard Mater. 2024 Sep 17:480:135873. doi: 10.1016/j.jhazmat.2024.135873.
Kaiyu Jiang 1,  Zhao Yin 2,  Wei Gong 3,  Yu-Xuan Liang 4,  Juncai Tu 3,  Xiaoya Tao 3,  Zhengqi Liu 3,  Yuanyuan Hu 3,  Jinjin Li 3,  Xiaoming Guo 3,  Juanying Ou 5,  Jie Zheng 6,  Beiwei Zhu 7,  Shiyi Ou 8
Affiliations

Affiliations

  • 1 Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China; Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National, Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen 518060, China; Shenzhen Key Laboratory of Food Nutrition and Health, College of Chemistry and Environmental Engineering and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, China.
  • 2 Department of Hematology, Guangdong Second Provincial General Hospital, Jinan University, Guangzhou 510317, China.
  • 3 Shenzhen Key Laboratory of Food Nutrition and Health, College of Chemistry and Environmental Engineering and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, China.
  • 4 Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National, Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen 518060, China; Shenzhen Key Laboratory of Food Nutrition and Health, College of Chemistry and Environmental Engineering and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, China.
  • 5 Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China; Institute of Food Safety & Nutrition, Jinan University, Guangzhou 510632, China.
  • 6 Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.
  • 7 Shenzhen Key Laboratory of Food Nutrition and Health, College of Chemistry and Environmental Engineering and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, China. Electronic address: zhubeiwei@163.com.
  • 8 Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China. Electronic address: tosy@jnu.edu.cn.
PMID: 39305594 DOI: 10.1016/j.jhazmat.2024.135873
Abstract

Acrolein (ACR) is a widespread, highly toxic substance that poses significant health risks. Flavonoids have been recognized as effective ACR scavengers, offering a possible way to reduce these risks. However, the lack of specific high-throughput screening methods has limited the identification of ACR scavengers, and their actual detoxifying capacity on ACR remains unknown. To address this, we developed a high-throughput screening platform to assess the ACR scavenging capacity of 322 Flavonoids. Our results showed that 80.7 % of the Flavonoids could scavenge ACR, but only 34.4 % exhibited detoxifying effects in an ACR-injured QSG7701 cell model. Some Flavonoids even increased toxicity. Structure-activity relationship (SAR) analysis indicated that galloyl and pyrogallol units enhance scavenging but worsen ACR-induced cytotoxicity. Further investigation revealed that epigallocatechin gallate (EGCG) could exacerbate ACR-induced redox disorder, leading to cell Apoptosis. Our findings provide crucial data on the scavenging and detoxifying capacities of 322 Flavonoids, highlighting that ACR scavengers might not mitigate ACR-induced toxicity and could pose additional safety risks.

Keywords

Acrolein; Detoxifying capacity; Flavonoids; High-throughput screening platform; Scavenging capacity.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z