2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Apoptosis Anti-infection
  3. Reactive Oxygen Species Endogenous Metabolite Apoptosis Ferroptosis Influenza Virus
  4. Acetylcysteine

Acetylcysteine  (Synonyms: 乙酰半胱氨酸; N-Acetylcysteine; N-Acetyl-L-cysteine; NAC)

目录号: HY-B0215 纯度: 99.44%
COA 产品使用指南

摩尔计算器

Acetylcysteine (N-Acetylcysteine) 是一种粘液溶解剂 (mucolytic agent)，可用于减少粘液的厚度。Acetylcysteine 是一种 ROS 抑制剂。Acetylcysteine 是半胱氨酸前体，通过中和花生四烯酸依赖的5-脂氧合酶活性所产生的毒性脂质来防止血红素诱导的铁中毒 (ferroptosis)。Acetylcysteine 可以诱导细胞凋亡 (apoptosis)，并具有抗流感病毒活性。此外，Acetylcysteine 是半胱氨酸在药物输送过程中最稳定形式，可用于双硫死亡 (disulfidptosis) 的研究。

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Acetylcysteine Chemical Structure

Acetylcysteine Chemical Structure

CAS No. : 616-91-1

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规格 价格 是否有货 数量
10 mM * 1 mL in Water ￥440
In-stock
500 mg ￥400
In-stock
5 g ￥500
In-stock
10 g ￥600
In-stock
50 g   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of Acetylcysteine:

Acetylcysteine 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 389 篇科研文献

WB

    Acetylcysteine purchased from MCE. Usage Cited in: Cell Death Discov. 2018 Apr 27;4:47.  [Abstract]

    Wild-type, Prx1KO, and Prx1OE cells are each divided into three groups, which are control groups, groups treated with 100 ng/mL LPS, and groups treated with 100 ng/mL LPS and NAC. Western blot analysis is then performed with antibodies against Bax, Bcl-2, Cyto-c, caspase 3, and GAPDH, and band intensity is measured with densitometry.

    Acetylcysteine purchased from MCE. Usage Cited in: Fundam Clin Pharmacol. 2017 Feb;31(1):64-74.  [Abstract]

    Advanced oxidation production product (AOPP)-triggered apoptosis-related signals are triggered by ROS production. Human chondrocytes are pretreated with N-Acetylcysteine (NAC; 5 mM) for 60 min, and then, chondrocyte are co-incubated with AOPP and NAC for 24 h. (a) Pretreatment of ROS scavenger NAC markedly alleviates AOPP-triggered mitochondria-mediated apoptosis proteins. (b) Pretreatment of chondrocyte with NAC abrogates the intracellular Ca2+ mobilization. (c) NAC pretreatment atte

    Acetylcysteine purchased from MCE. Usage Cited in: Sci Rep. 2016 Oct 27;6:35995.  [Abstract]

    Pretreatment of NAC, a free radical scavenger, largely decreases the expression of caspase-3 and almost abolishes the H2O2 induced upregulation of PRX1. Western blot analysis shows the pretreatment of osteoblast cells with E2 partially abrogates the increased PRX1 and caspase-3 expressions induced by H2O2.

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Acetylcysteine (N-Acetylcysteine) is a mucolytic agent which reduces the thickness of the mucus. Acetylcysteine is a ROS inhibitor. Acetylcysteine is a cysteine precursor, prevents hemin-induced ferroptosis by neutralizing toxic lipids generated by arachidonate-dependent activity of 5-lipoxygenases. Acetylcysteine induces cell apoptosis. Acetylcysteine also has anti-influenza virus activities. In addition, Acetylcysteine ​​is the most stable form of cysteine ​​during drug delivery and can be used in disulfidptosis studies[1][2][3][4][5][6][7][8].

    IC50 & Target

    Human Endogenous Metabolite

     

    体外研究
    (In Vitro)

    Acetylcysteine 可防止凋亡 DNA 片段化，并在缺乏其他营养支持的情况下维持血清剥夺 PC12 细胞的长期存活。Acetylcysteine 还可防止 PC12 细胞和交感神经元死亡[2]。
    Acetylcysteine 导致大鼠和人主动脉平滑肌细胞的活力呈剂量依赖性降低[3]。
    Acetylcysteine 激活 PC12 细胞中的 Ras 细胞外信号调节激酶 (ERK) 通路。Acetylcysteine 保护神经元细胞免于因营养支持撤消而引起的死亡。Acetylcysteine 增加血管组织中蛋白质结合储存的一氧化氮 (NO) 释放。Acetylcysteine 预处理 PC12 细胞会干扰 NGF 依赖的信号传导和神经突的生长，提示 Acetylcysteine 会干扰 NGF 机制中的氧化还原敏感步骤[4]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Acetylcysteine 相关抗体:
    体内研究
    (In Vivo)

    Acetylcysteine (150，300 mg/kg) 处理显著降低所有处理组的肝转氨酶，主要是 Acetylcysteine 300 mg/kg 组。肺谷胱甘肽过氧化物酶在 Acetylcysteine 300 mg/kg 组中显著增加 (P=0.04)，而其他氧化生物标志物显示无显著性差异[6]。
    Acetylcysteine 改善 12 个月大的 SAMP8 小鼠在 T 迷宫电击回避范例和杠杆按压食欲任务中的认知，而不会对运动产生非特异性影响活动、避免电击的动机或体重[5]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    163.20

    Formula

    C5H9NO3S
    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    乙酰半胱氨酸
    结构分类
    初始来源
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶解性数据
    细胞实验： 

    H2O 中的溶解度 : 100 mg/mL (612.75 mM; 超声助溶)

    DMSO 中的溶解度 : 100 mg/mL (612.75 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 6.1275 mL 30.6376 mL 61.2753 mL
    5 mM 1.2255 mL 6.1275 mL 12.2551 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 120 mg/mL (735.30 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.44%

    COA (286 KB) HNMR (123 KB) CNMR (134 KB) RP-HPLC (277 KB) MS (69 KB)

    产品使用指南 (1538 KB)
    参考文献
    Cell Assay
    [2]

    For survival experiments, washed cells are resuspended in RPM1 1640 medium and plated in 0.5 mL at a density of 8-10×105 per well in 24 well plastic culture dishes coated with rat tail collagen. To feed, but to avoid loss of floating cells, fresh medium (0.2 mL) is added to the cultures on days 1, 5, and 10. For experiments involving "primed" PC12 cells, cultures are pretreated for l-2 weeks with NGF in RPM1 1640 medium supplemented with 1% heat-iN-acetylcysteinetivated horse serum. The cells are then washed and passaged into serum-free RPM1 1640 medium.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [6]

    Rats: Rats are randomLy allocated into five groups: sham group (n=5), control group with IIR (n=8) and three groups with IIR who are given Acetylcysteine in different dosages: 150 mg/kg intraperitoneally 5 min before ischemia (n=8, group Acetylcysteine 150), 300 mg/kg i.p 5 min before ischemia (n=7, group Acetylcysteine 300), and 150 mg/kg i.p 5 min before ischemia plus 150 mg/kg 5 min before reperfusion (n=7, group Acetylcysteine 150 + 150). After 4 h of reperfusion, the animals are euthanized by exsanguination from the abdominal aorta.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    Acetylcysteine 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 6.1275 mL 30.6376 mL 61.2753 mL 153.1882 mL
    5 mM 1.2255 mL 6.1275 mL 12.2551 mL 30.6376 mL
    10 mM 0.6128 mL 3.0638 mL 6.1275 mL 15.3188 mL
    15 mM 0.4085 mL 2.0425 mL 4.0850 mL 10.2125 mL
    20 mM 0.3064 mL 1.5319 mL 3.0638 mL 7.6594 mL
    25 mM 0.2451 mL 1.2255 mL 2.4510 mL 6.1275 mL
    30 mM 0.2043 mL 1.0213 mL 2.0425 mL 5.1063 mL
    40 mM 0.1532 mL 0.7659 mL 1.5319 mL 3.8297 mL
    50 mM 0.1226 mL 0.6128 mL 1.2255 mL 3.0638 mL
    60 mM 0.1021 mL 0.5106 mL 1.0213 mL 2.5531 mL
    80 mM 0.0766 mL 0.3830 mL 0.7659 mL 1.9149 mL
    100 mM 0.0613 mL 0.3064 mL 0.6128 mL 1.5319 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Acetylcysteine616-91-1N-Acetylcysteine N-Acetyl-L-cysteine NAC乙酰半胱氨酸Reactive Oxygen SpeciesEndogenous MetaboliteApoptosisFerroptosisInfluenza VirusInhibitorinhibitorinhibit

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