2. Academic Validation
  3. IL-1β-induced epithelial cell and fibroblast transdifferentiation promotes neutrophil recruitment in chronic rhinosinusitis with nasal polyps

IL-1β-induced epithelial cell and fibroblast transdifferentiation promotes neutrophil recruitment in chronic rhinosinusitis with nasal polyps

  • Nat Commun. 2024 Oct 22;15(1):9101. doi: 10.1038/s41467-024-53307-0.
Xinyu Xie # 1 2,  Pin Wang # 1 2,  Min Jin # 3,  Yue Wang # 4,  Lijie Qi 1 2,  Changhua Wu 1,  Shu Guo 1,  Changqing Li 1,  Xiaojun Zhang 1 2,  Ye Yuan 1,  Xinyi Ma 1,  Fangying Liu 1,  Weiyuan Liu 1,  Heng Liu 1,  Chen Duan 1 2,  Ping Ye 1 2,  Xuezhong Li 1 2,  Larry Borish 5 6,  Wei Zhao 7 8,  Xin Feng 9 10
Affiliations

Affiliations

  • 1 Department of Otorhinolaryngology, National Health Commission Key Laboratory of Otorhinolaryngology, Qilu Hospital of Shandong University, Jinan, China.
  • 2 Shandong Provincial Key Medical and Health Discipline, Qilu Hospital of Shandong University, Jinan, China.
  • 3 Department of Anesthesiology, Qilu Hospital of Shandong University, Jinan, China.
  • 4 Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan, China.
  • 5 Departments of Medicine, University of Virginia Health System, Charlottesville, VA, USA.
  • 6 Departments of Microbiology, University of Virginia Health System, Charlottesville, VA, USA.
  • 7 Key Laboratory for Experimental Teratology of the Chinese Ministry of Education, School of Basic Medical Science, Shandong University, Jinan, China.
  • 8 Key Laboratory of Infection and Immunity of Shandong Province, School of Basic Medical Science, Shandong University, Jinan, China.
  • 9 Department of Otorhinolaryngology, National Health Commission Key Laboratory of Otorhinolaryngology, Qilu Hospital of Shandong University, Jinan, China. drfengxin@sdu.edu.cn.
  • 10 Shandong Provincial Key Medical and Health Discipline, Qilu Hospital of Shandong University, Jinan, China. drfengxin@sdu.edu.cn.
  • # Contributed equally.
PMID: 39438439 DOI: 10.1038/s41467-024-53307-0
Abstract

Neutrophilic inflammation contributes to multiple chronic inflammatory airway diseases, including asthma and chronic rhinosinusitis with nasal polyps (CRSwNP), and is associated with an unfavorable prognosis. Here, using single-cell RNA Sequencing (scRNA-seq) to profile human nasal mucosa obtained from the inferior turbinates, middle turbinates, and nasal polyps of CRSwNP patients, we identify two IL-1 signaling-induced cell subsets-LY6D+ club cells and IDO1+ fibroblasts-that promote neutrophil recruitment by respectively releasing S100A8/A9 and CXCL1/2/3/5/6/8 into inflammatory regions. IL-1β, a pro-inflammatory cytokine involved in IL-1 signaling, induces the transdifferentiation of LY6D+ club cells and IDO1+ fibroblasts from primary epithelial cells and fibroblasts, respectively. In an LPS-induced neutrophilic CRSwNP mouse model, blocking IL-1β activity with a receptor antagonist significantly reduces the numbers of LY6D+ club cells and IDO1+ fibroblasts and mitigates nasal inflammation. This study implicates the function of two cell subsets in neutrophil recruitment and demonstrates an IL-1-based intervention for mitigating neutrophilic inflammation in CRSwNP.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z