2. Academic Validation
  3. Anti-inflammatory effect of polysaccharides from Sambucus williamsii Hance roots in lipopolysaccharide-stimulated RAW264.7 macrophages and acute lung injury in mice

Anti-inflammatory effect of polysaccharides from Sambucus williamsii Hance roots in lipopolysaccharide-stimulated RAW264.7 macrophages and acute lung injury in mice

  • Int J Biol Macromol. 2025 Feb 21;306(Pt 1):141368. doi: 10.1016/j.ijbiomac.2025.141368.
Xianqun Meng 1,  Qing Wei 2,  Siyi Wang 3,  Shanshan Liang 4,  Dan Wang 5,  Haixue Kuang 6,  Qiuhong Wang 7,  Tian Xie 8
Affiliations

Affiliations

  • 1 College of Traditional Chinese Medicine, Wenzhou Medical University, Wenzhou 325000, China.
  • 2 Division of Life Science, State Key Laboratory of Molecular Neuroscience, Molecular Neuroscience Center, The Hong Kong University of Science and Technology, 999077, China.
  • 3 Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China.
  • 4 Plant Polysaccharide Research Center, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China.
  • 5 Department of Traditional Chinese Medicine Identification, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
  • 6 Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China. Electronic address: khxandwqh@163.com.
  • 7 Key Laboratory of Chinese Medicinal Herbs Preparation, Guangdong Pharmaceutical University, Guangdong 510000, China. Electronic address: qhwang668@sina.com.
  • 8 College of Traditional Chinese Medicine, Wenzhou Medical University, Wenzhou 325000, China. Electronic address: tianxie@hznu.edu.cn.
PMID: 39988171 DOI: 10.1016/j.ijbiomac.2025.141368
Abstract

This study aimed to investigate the structural characterization of polysaccharide from Sambucus williamsii Hance roots (SWH1-1) and its effects on inflammation and acute lung injury (ALI). Results showed that SWH1-1 had a molecular weight of 10,040 Da and primarily consisted of arabinose, galactose, glucose and mannose. Its main backbone was composed of →6)-α-D-Glcp-(1→, →6)-α-D-Man-(1→, and →6)-β-D-Galp-(1→. Besides, SWH1-1 could alleviate nitric oxide (NO), tumour necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) contents in lipopolysaccharide (LPS)-treated RAW 264.7 cells. In mice experiment, SWH1-1 alleviated LPS-induced lung injury by reversing lung histological changes, inhibiting Apoptosis, and reducing myeloperoxidase (MPO) levels. SWH1-1 also reduced TNF-α, IL-6 and IL-1β values in vivo. SWH1-1 reduced oxidative stress by upregulating superoxide dismutase (SOD), glutathione (GSH) and downregulating malondialdehyde (MDA) contents. Moreover, SWH1-1 inhibited the activation of the Toll-like Receptor 4 (TLR4)/nuclear factor κB (NF-κB)/mitogen-activated protein kinase (MAPK) pathway in LPS-injured lung tissues. The results of RAW264.7 cell experiments further verified that SWH1-1 inhibited TLR4/NF-κB/MAPK pathway. Our research displayed that SWH1-1 improved LPS-induced ALI by alleviating inflammation, oxidative stress and TLR4/NF-κB/MAPK pathway. These results suggest that SWH1-1 is a potential anti-inflammatory candidate for the treatment of LPS-induced acute lung injury.

Keywords

Acute lung injury; Polysaccharides from Sambucus williamsii Hance roots; TLR4/NF-κB/MAPK signaling pathway.

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