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  4. N-Aminofluorescein

N-Aminofluorescein 

目录号: HY-D1601 纯度: 99.91%
COA 产品使用指南

摩尔计算器

N-Aminofluorescein 是一种螺旋形荧光素酰肼，是一种对 Cu2+ 具有高度选择性和敏感性的荧光探针。N-Aminofluorescein 对其他常见金属离子无选择性荧光响应，可用于生物体系中 Cu2+ 的直接检测 (λex/em=495/516 nm)。

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N-Aminofluorescein Chemical Structure

N-Aminofluorescein Chemical Structure

CAS No. : 98907-26-7

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规格 价格 是否有货 数量
5 mg ￥410
In-stock
10 mg ￥650
In-stock
25 mg ￥1300
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

N-Aminofluorescein 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

N-Aminofluorescein is a fluorescein hydrazide with spiro form, a highly selective and sensitive fluorescence probe for Cu2+. N-Aminofluorescein has no selective fluorescence response to other common metal ions, can be used for direct detection of Cu2+ in biological systems with λex/em=495/516 nm[1][2].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
N-Aminofluorescein consists of a fluorescein moiety and a hydrazide group to recognize and bind Cu2+, can promote the hydrolysis of amide[1].
N-Aminofluorescein (FG) shows selectivity on Cu2+ and shows the absorption and emission bands at 632 nm and 515 nm in 70% aqueous HEPES buffered solution (pH 7.4) containing Cu2+[2].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
General procedure for Cu2+ determination[1]:
1.Prepare the 1.0 mM stock solution of spiro form fluorescein hydrazide in ethanol;
2.Conduct the fluorescence-on reaction in 0.01 M Tris-HCl buffer (pH 7.2), with 10 μM N-Aminofluorescein;
3.Add an appropriate volume of sample solution with a final Cu2+ concentration of not more than 10 μM, and adjust the final volume 10 mL with 0.01 M Tris-HCl buffer (pH 7.2);
4.After 2 h, transfer a 3-mL portion of the solution to a 1-cm quartz cell, and measure the fluorescence intensity/spectrum at room temperature with λex/em = 495/516 nm and both excitation and emission slit widths of 5 nm;?
5.In the meantime, prepare a blank solution containing no Cu2+ and measure with the same conditions for comparison.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

N-Aminofluorescein 相关抗体:
分子量

346.34

Formula

C20H14N2O4
CAS 号
性状

固体

颜色

Off-white to light yellow

Emission (Em)

516
Excitation (Ex)

495
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 125 mg/mL (360.92 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.8873 mL 14.4367 mL 28.8734 mL
5 mM 0.5775 mL 2.8873 mL 5.7747 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.91%

COA (178 KB) HNMR (255 KB) LCMS (91 KB)

产品使用指南 (1538 KB)
参考文献

N-Aminofluorescein 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8873 mL 14.4367 mL 28.8734 mL 72.1834 mL
5 mM 0.5775 mL 2.8873 mL 5.7747 mL 14.4367 mL
10 mM 0.2887 mL 1.4437 mL 2.8873 mL 7.2183 mL
15 mM 0.1925 mL 0.9624 mL 1.9249 mL 4.8122 mL
20 mM 0.1444 mL 0.7218 mL 1.4437 mL 3.6092 mL
25 mM 0.1155 mL 0.5775 mL 1.1549 mL 2.8873 mL
30 mM 0.0962 mL 0.4812 mL 0.9624 mL 2.4061 mL
40 mM 0.0722 mL 0.3609 mL 0.7218 mL 1.8046 mL
50 mM 0.0577 mL 0.2887 mL 0.5775 mL 1.4437 mL
60 mM 0.0481 mL 0.2406 mL 0.4812 mL 1.2031 mL
80 mM 0.0361 mL 0.1805 mL 0.3609 mL 0.9023 mL
100 mM 0.0289 mL 0.1444 mL 0.2887 mL 0.7218 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-Aminofluorescein98907-26-7Fluorescent Dyespiro formfluorescein hydrazideCuselective fluorescence responsebiological systemfluoresceingalactoseoptical probecopperInhibitorinhibitorinhibit

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