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  4. Naph-EA-mal

Naph-EA-mal  (Synonyms: Thiol-green 1)

目录号: HY-D1261 纯度: ≥95.0%
COA 产品使用指南

摩尔计算器

Naph-EA-mal (Thiol-green 1) 是一种快速检测和超快 turn-on 型硫醇 (thiol) 荧光探针，可用于蛋白标记和生物成像。Naph-EA-mal (Thiol-green 1) 可用于检测活细胞中的硫醇，标记蛋白质硫醇，定量细胞裂解液中总硫醇的浓度，并确定固定细胞中可逆的蛋白质硫醇氧化。Ex: 488 nM; Em: 540 nM。

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Naph-EA-mal Chemical Structure

Naph-EA-mal Chemical Structure

CAS No. : 210292-65-2

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3.  试用装只面向终端客户。

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥3300
In-stock
1 mg ￥1363
In-stock
5 mg ￥3000
In-stock
10 mg ￥4800
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

Naph-EA-mal 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Naph-EA-mal (Thiol-green 1) is a rapid detect and ultrafast turn-on thiol fluorescence probe for protein labeling and bioimaging. Naph-EA-mal (Thiol-green 1) can be used to detect thiols in living cells, label the protein thiols, quantify the concentration of total thiols in cell lysate, and determine the reversible protein thiols oxidation in fixed cells[1]. Ex: 488 nM; Em: 540 nM.

体外研究
(In Vitro)

Imaging thiols in living cells[1]
Hep G2 cells incubated with Naph-EA-mal (Thiol-green 1) (1 μM;5 mins), and strong green fluorescence can be observed. When cells are preincubated the cells to block the intracellular thiols with NEM (a thiol block reagent; 100 μM), a weaker fluorescence is observed in cells[1].
Monitoring thiol-disulfide levels in fixed cells
Due to the majority of cellular thiols are present in the reduced form, Naph-EA-mal (Thiol-green 1) (10 μM) induces strong fluorescence in Hep G2 cells. After diamide treatment, fluorescent signal sharp dimed is observed[1].
The Hep G2 cells, oxidized by diamide (200 μM; 30 min), are fixed by 70% EtOH and the remaining free thiols are blocked by NEM (100 μM). then The cells are further treated with TCEP (5 mM) followed by addition of the Naph-EA-mal (Thiol-green 1) (10 μM). The green fluorescent signal is detected again[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Naph-EA-mal 相关抗体:
分子量

391.42

Formula

C22H21N3O4
CAS 号
性状

固体

颜色

Light yellow to orange

Emission (Em)

540
Excitation (Ex)

488
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 33.33 mg/mL (85.15 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.5548 mL 12.7740 mL 25.5480 mL
5 mM 0.5110 mL 2.5548 mL 5.1096 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
染色示例
参考文献

Naph-EA-mal 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5548 mL 12.7740 mL 25.5480 mL 63.8700 mL
5 mM 0.5110 mL 2.5548 mL 5.1096 mL 12.7740 mL
10 mM 0.2555 mL 1.2774 mL 2.5548 mL 6.3870 mL
15 mM 0.1703 mL 0.8516 mL 1.7032 mL 4.2580 mL
20 mM 0.1277 mL 0.6387 mL 1.2774 mL 3.1935 mL
25 mM 0.1022 mL 0.5110 mL 1.0219 mL 2.5548 mL
30 mM 0.0852 mL 0.4258 mL 0.8516 mL 2.1290 mL
40 mM 0.0639 mL 0.3194 mL 0.6387 mL 1.5968 mL
50 mM 0.0511 mL 0.2555 mL 0.5110 mL 1.2774 mL
60 mM 0.0426 mL 0.2129 mL 0.4258 mL 1.0645 mL
80 mM 0.0319 mL 0.1597 mL 0.3194 mL 0.7984 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Naph-EA-mal210292-65-2Thiol-green 1Fluorescent DyeNEMthiol-disulfidethiolintracellularInhibitorinhibitorinhibit

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