Pacanalotamab  (Synonyms: AMG 420; BI-836909)

Pacanalotamab (AMG 420; BI-836909) is a bispecific T-cell engager (BiTE) targeting to BCMA and CD3E. BCMA refers to B cell maturation antigen, as Pacanalotamab redirecting T cells to BCMA expressing cells on the cell surface. Pacanalotamab conducts T-cell redirected lysis of human multiple myeloma (MM) cell lines^[1].

(scFv-heavy-kappa)-(scFv-heavy-lambda)

Chimeric; Humanized

B cell maturation antigen, BCMA; CD3ɛ^[1]

Pacanalotamab (1 pg/mL-100 ng/mL；24 h) 通过 T 细胞激活诱导 BCMA 依赖性肿瘤细胞裂解^[1]。Pacanalotamab 诱导人多发性骨髓瘤细胞系的剂量依赖性重定向裂解，EC₉₀ 值范围为 16-810 pg/mL^[1]。
Pacanalotamab (100 ng/mL；24 小时) 诱导磷脂酰丝氨酸从质膜内层转移到外层，导致 MM.1R 细胞凋亡^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Pacanalotamab (1 pg/mL-100 ng/mL；24 h) 通过 T 细胞激活诱导 BCMA 依赖性肿瘤细胞裂解^[1]。Pacanalotamab 诱导人多发性骨髓瘤细胞系的剂量依赖性重定向裂解，EC₉₀ 值范围为 16-810 pg/mL^[1]。
Pacanalotamab (100 ng/mL；24 小时) 诱导磷脂酰丝氨酸从质膜内层转移到外层，导致 MM.1R 细胞凋亡^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

2251756-52-0

液体

Colorless to light yellow

Shipping with dry ice.

Please store the product under the recommended conditions in the Certificate of Analysis.

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  (scFv-heavy-kappa)-(scFv-heavy-lambda)

• 
  Immobilized Pacanalotamab can bind Human BCMA Protein (ECD, hFc Tag). The EC₅₀ for this effect is 0.597 ng/mL.
• 
  Immobilized Pacanalotamab can bind Human CD3 epsilon/CD3e Protein (ECD, hFc Tag). The EC₅₀ for this effect is 1.506 ng/mL.

• [1]. Hipp S, et al. A novel BCMA/CD3 bispecific T-cell engager for the treatment of multiple myeloma induces selective lysis in vitro and in vivo. Leukemia. 2017 Aug;31(8):1743-1751.  [Content Brief]