1. Epigenetics TGF-beta/Smad
  2. PKC
  3. Protein Kinase C (530-558)

Protein Kinase C (530-558)  (Synonyms: PKC fragment (530-558))

目录号: HY-P1288
产品使用指南 技术支持

Protein Kinase C (530-558) 是蛋白激酶C (PKC) 的肽片段,是一种有效的 PKC 激活剂。Protein Kinase C (530-558) 可显著抑制破骨细胞骨吸收。

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Custom Peptide Synthesis

Protein Kinase C (530-558) Chemical Structure

Protein Kinase C (530-558) Chemical Structure

CAS No. : 122613-29-0

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 是否有货
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100 mg   询价  
250 mg   询价  

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Customer Review

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Protein Kinase C (530-558), a peptide fragment of protein kinase C (PKC), is a potent PKC activator. Protein Kinase C (530-558) significantly inhibits osteoclastic bone resorption[1].

体外研究
(In Vitro)

Protein Kinase C (530-558) (1 nM-1 μM, 24 h) causes a dose-responsive inhibition of bone resorption, which is accompanied by a rapid and distinctive change in osteoclast morphology[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Osteoclast cell
Concentration: 1 nM, 10 nM, 100 nM, 1 μM
Incubation Time: 24 h
Result: Showed a dose-responsive, marked inhibition of bone resorption was observed between 10 nM and 1 μM. At 1 μM, resorption was inhibited by more thaln 87 %.
分子量

3354.67

Formula

C148H221N35O50S2

CAS 号
Sequence Shortening

LLYEMLAGQAPFEGEDEDELFQSIMEHNV-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
细胞实验: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
纯度 & 产品资料
参考文献
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Protein Kinase C (530-558)
目录号:
HY-P1288
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