1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. 15-PGDH/HPGD 蛋白, Human (His)

15-PGDH/HPGD 蛋白, Human (His)

目录号: HY-P75547
产品使用指南

15-PGDH/HPGD 是一种短链非金属酶乙醇脱氢酶,对于前列腺素代谢至关重要,影响炎症等各种生理和细胞过程。突变导致肥厚性骨关节病。在膀胱 (RPKM 176.8) 和胃 (RPKM 67.9) 等组织中存在偏向表达。多种同工型源自不同的转录变体。15-PGDH/HPGD 蛋白, Human (His) 是重组的 15-PGDH/HPGD 蛋白,由 E. coli 表达,带有 C-His 标签。

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描述

15-PGDH/HPGD, a short-chain nonmetalloenzyme alcohol dehydrogenase, is crucial for prostaglandin metabolism, influencing various physiological and cellular processes like inflammation. Mutations lead to hypertrophic osteoarthropathy. Biased expression in urinary bladder (RPKM 176.8) and stomach (RPKM 67.9), among other tissues. Multiple isoforms arise from various transcript variants. 15-PGDH/HPGD Protein, Human (His) is the recombinant human-derived 15-PGDH/HPGD protein, expressed by E. coli , with C-His labeled tag.

研究背景

15-PGDH/HPGD 蛋白是短链非金属酶乙醇脱氢酶蛋白家族的成员,在前列腺素的代谢中发挥着至关重要的作用,有助于各种生理和细胞过程,包括炎症。该基因的突变与原发性常染色体隐性遗传肥大性骨关节病和颅骨骨关节病相关,强调了其在维持正常骨骼发育中的重要性。该基因表现出多种转录变体,编码不同的亚型,增加了所编码酶的功能多样性。值得注意的是,15-PGDH/HPGD 表现出偏向表达,在膀胱 (RPKM 176.8)、胃 (RPKM 67.9) 和 11 个其他组织中观察到水平升高,表明其在这些组织中的特殊作用及其在不同生理环境中的潜在参与。

种属

Human

表达系统

E. coli

标签

C-His

蛋白编号

P15428-1/NP_000851.2 (M1-Q266)

基因 ID
蛋白结构
N-term
15-PGDH (M1-Q266)
Accession # P15428-1/NP_000851.2
His
C-term
同用名
15-hydroxyprostaglandin dehydrogenase [NAD(+)]; HPGD; PGDH1; SDR36C1
分子量

Approximately 27 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

15-PGDH/HPGD Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
15-PGDH/HPGD Protein, Human (His)
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HY-P75547
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