1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Acetylcholinesterase/ACHE 蛋白, Mouse (HEK293, His)

Acetylcholinesterase/ACHE 蛋白, Mouse (HEK293, His)

目录号: HY-P72811
COA 产品使用指南

乙酰胆碱酯酶 (ACHE) 蛋白表现出多种功能,包括乙酰胆碱酯酶活性和蛋白质自缔合。它调节乙酰胆碱加工、受体信号传导和回收。Acetylcholinesterase/ACHE 蛋白, Mouse (HEK293, His) 是重组的 乙酰胆碱酯酶/ACHE 蛋白,由 HEK293 表达,带有 C-10*His 标签。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥980 In-stock
10 μg ¥1550 In-stock
20 μg ¥2500 In-stock
50 μg ¥4750 询价
100 μg ¥7600 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Acetylcholinesterase (ACHE) proteins exhibit multiple functions, including acetylcholinesterase activity and protein self-association. It regulates acetylcholine processing, receptor signaling and recycling. Acetylcholinesterase/ACHE Protein, Mouse (HEK293, His) is the recombinant mouse-derived Acetylcholinesterase/ACHE protein, expressed by HEK293 , with C-10*His labeled tag.

研究背景

乙酰胆碱酯酶 (ACHE) 蛋白具有多种功能,表现出乙酰胆碱酯酶活性、相同的蛋白结合活性以及蛋白自缔合能力。它参与冷诱导产热的积极调节,使其处于关键过程的上游,例如乙酰胆碱分解代谢过程、乙酰胆碱受体信号传导途径和受体再循环的调节。ACHE 存在于多种细胞成分中,包括基底膜、细胞表面和神经肌肉接头。它的表达跨越多个结构,包括消化系统、泌尿生殖系统、肌肉组织、神经系统和感觉器官。值得注意的是,ACHE 的人类直系同源物与阿尔茨海默病、癫痫和重症肌无力等重要健康状况有关,凸显了该蛋白质在各种生理环境中的重要性。广泛的表达模式进一步强调了其在多种组织中的基本作用,特别是在小脑和胸腺中。

生物活性

Measured by its ability to cleave Acetylthiocholine. The specific activity is > 250 nmoL/min/μg, as measured under the described conditions.

种属

Mouse

表达系统

HEK293

标签

C-10*His

蛋白编号

P21836-1/NP_033729.1 (E32-L614)

基因 ID
蛋白结构
N-term
ACHE (E32-L614)
Accession # P21836-1/NP_033729.1
10*His
C-term
同用名
Acetylcholinesterase; AChE
氨基酸序列

EGREDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGVLDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPASPTPVLIWIYGGGFYSGAASLDVYDGRFLAQVEGAVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLALQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRATLLARLVGCPPGGAGGNDTELIACLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPEALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPEDPTHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPLDPSLNYTTEERIFAQRLMKYWTNFARTGDPNDPRDSKSPQWPPYTTAAQQYVSLNLKPLEVRRGLRAQTCAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQERCSDL

分子量

Approximately 80 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Acetylcholinesterase/ACHE Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Acetylcholinesterase/ACHE Protein, Mouse (HEK293, His)
目录号:
HY-P72811
需求量: