ACPP/Prostatic Acid Phosphatase 蛋白, Mouse (HEK293, His)

The acid prostatic phosphatase (ACPP) protein, encapsulated in virus-like particles (VLPs), is an acid tyrosine phosphatase that skillfully dephosphorylates a variety of substrates. ACPP-VLP possesses lipid phosphatase activity and inactivates lysophosphatidic acid. ACPP/Prostatic Acid Phosphatase Protein, Mouse (HEK293, His) is the recombinant mouse-derived ACPP/Prostatic Acid Phosphatase protein, expressed by HEK293 , with C-His labeled tag. The total length of ACPP/Prostatic Acid Phosphatase Protein, Mouse (HEK293, His) is 350 a.a., with molecular weight of 44-55 kDa.

前列腺酸性磷酸酶 (ACPP) 蛋白是一种多功能非特异性酪氨酸磷酸酶，在酸性条件 (pH 4-6) 下对多种底物表现出催化活性，包括烷基、芳基和酰基正磷酸单酯，以及磷酸化蛋白质。值得注意的是，ACPP 还表现出脂质磷酸酶活性，有助于精浆中溶血磷脂酸的失活。除了其酪氨酸磷酸酶功能外，ACPP 在背根神经节神经元中还具有 5'-核酸外切酶活性，从而导致 AMP 产生腺苷。反过来，这种细胞外腺苷通过激活伤害性神经元中的 A1R 有助于减轻慢性疼痛。ACPP 的多方面酶活性凸显了其在各种细胞过程中的潜在意义及其治疗意义，特别是在疼痛管理方面。

Measured by its ability to cleave 2mM substrate p-Nitrophenyl phosphate (pNPP). which can be measured by absorbance at 410 nm that incubate at room temperature for 5 minutes. The specific activity is 104600.068 pmol/min/µg, as measured under the described conditions.

Mouse

HEK293

C-6*His

Q8CE08 (K32-R381)

56318  [NCBI]

KELKFVTLVFRHGDRGPIETFPTDPITESSWPQGFGQLTQWGMEQHYELGSYIRKRYGRFLNDTYKHDQIYIRSTDVDRTLMSAMTNLAALFPPEGISIWNPRLLWQPIPVHTVSLSEDRLLYLPFRDCPRFEELKSETLESEEFLKRLHPYKSFLDTLSSLSGFDDQDLFGIWSKVYDPLFCESVHNFTLPSWATEDAMIKLKELSELSLLSLYGIHKQKEKSRLQGGVLVNEILKNMKLATQPQKYKKLVMYSAHDTTVSGLQMALDVYNGVLPPYASCHMMELYHDKGGHFVEMYYRNETQNEPYPLTLPGCTHSCPLEKFAELLDPVISQDWATECMATSSHQVLR

Approximately 44-55 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.