ADAM17/TACE 蛋白, Mouse (HEK293, His)

The ADAM17/TACE protein cleaves TNF-α, releasing it from the membrane and activating various cell surface proteins such as JAM3, ACE2, and the p75 TNF receptor. It also triggers the Notch pathway by cleaving Notch into membrane-associated fragments. ADAM17/TACE Protein, Mouse (HEK293, His) is the recombinant mouse-derived ADAM17/TACE protein, expressed by HEK293 , with C-His labeled tag. The total length of ADAM17/TACE Protein, Mouse (HEK293, His) is 546 a.a..

ADAM17/TACE 蛋白负责裂解 TNF-α 的膜结合前体，将其转化为成熟的可溶形式。它还在各种细胞表面蛋白的蛋白水解释放中发挥着至关重要的作用，包括 JAM3、ACE2、p75 TNF 受体、II 型白细胞介素 1 受体、p55 TNF 受体、转化生长因子-α、L-选择素、生长激素受体、MUC1 和淀粉样前体蛋白。此外，ADAM17/TACE 通过介导 Notch 裂解来激活 Notch 通路，生成称为 Notch 细胞外截短 (NEXT) 的膜相关中间片段。在止血过程中，它通过脱落血小板糖蛋白 Ib α 链 (GP1BA) 发挥作用，同时还促进蛋白水解裂解和分泌形式的 LAG3 和 IL6R 的释放。此外，ADAM17/TACE 在刺激自然杀伤细胞时介导 FCGR3A 的蛋白水解裂解和脱落，从而增强 NK 细胞的运动性和与调理靶细胞的分离。

Measured by its ability to cleave 10μM fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 1818.15 pmol/min/µg.

Mouse

HEK293

C-6*His

Q9Z0F8 (P18-D563)

11491  [NCBI]

RPPEEAGSGSHPRLEKLDSLLSDYDILSLANIQQHSIRKRDLQSATHLETLLTFSALKRHFKLYLTSSTERFSQNLRVVVVDGKEESEYSVKWQNFFSGHVVGEPDSRVLAHIGDDDVTVRINTDGAEYNVEPLWRFVNDTKDKRMLVYKSEDIKDFSRLQSPKVCGYLNADSEELLPKGLIDREPSEEFVRRVKRPRPPEEAGSGSHPRLEKLDSLLSDYDILSLANIQQHSIRKRDLQSATHLETLLTFSALKRHFKLYLTSSTERFSQNLRVVVVDGKEESEYSVKWQNFFSGHVVGEPDSRVLAHIGDDDVTVRINTDGAEYNVEPLWRFVNDTKDKRMLVYKSEDIKDFSRLQSPKVCGYLNADSEELLPKGLIDREPSEEFVRRVKRRAEPNPLKNTCKLLVVADHRFYKYMGRGEESTTTNYLIELIDRVDDIYRNTSWDNAGFKGYGVQIEQIRILKSPQEVKPGERHFNMAKSFPNEEKDAWDVKMLLEQFSFDIAEEASKVCLAHLFTYQDFDMGTLGLAYVGSPRANSHGGVCPKAYYNPTVKKNIYLNSGLTSTKNYGKTILTKEADLVTTHELGHNFGAEHDPDGLAECAPNEDQGGKYVMYPIAVSGDHENNKMFSNCSKQSIYKTIESKAQECFQERSNKVCGNSRVDEGEECDPGIMYLNNDTCCNSDCTLKPGVQCSDRNSPCCKNCQFETAQKKCQEAINATCKGVSYCTGNSSECPPPGDAED

Approximately 55-75 & 25 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.