2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. ADH7 蛋白, Human (HEK293, His)

ADH7 蛋白, Human (HEK293, His)

目录号: HY-P7473
产品使用指南

ADH7 蛋白, Human (HEK293, His)是在 C 端带有 His 标签的人重组 ADH7，通过哺乳动物表达系统表达，并表达编码 Met1-Phe386 的靶基因。

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规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

ADH7 Protein, Human (HEK293, His) is human recombinant ADH7 with His tag at the C-terminus. ADH7 Protein, Human (HEK293, His) is expressed by Mammalian expression system and the target gene encoding Met1-Phe386 is expressed.
研究背景

ADH7是一种NADPH依赖性酶，属于中链醇脱氢酶超家族。ADH7 受 miR-3065 调节，并且 miR-3065 与 LINC01133 相互作用。ADH7在视黄酸代谢过程和视黄醇代谢途径中富集。ADH7 与宫颈癌存活率显着相关[1][2]。
种属

Human
表达系统

HEK293
标签

C-6*His
蛋白编号

P40394 (M1-F386)
基因 ID

131  [NCBI]
蛋白结构
N-term
ADH7 (M1-F386)
Accession # P40394
6*His
C-term
中文名
重组人 ADH7，His (HEK293 细胞表达)
同用名
rHuADH7, His; Alcohol dehydrogenase class 4 mu, sigma chain; ADH-7
氨基酸序列

MFAEIQIQDKDRMGTAGKVIKCKAAVLWEQKQPFSIEEIEVAPPKTKEVRIKILATGICRTDDHVIKGTMVSKFPVIVGHEATGIVESIGEGVTTVKPGDKVIPLFLPQCRECNACRNPDGNLCIRSDITGRGVLADGTTRFTCKGKPVHHFMNTSTFTEYTVVDESSVAKIDDAAPPEKVCLIGCGFSTGYGAAVKTGKVKPGSTCVVFGLGGVGLSVIMGCKSAGASRIIGIDLNKDKFEKAMAVGATECISPKDSTKPISEVLSEMTGNNVGYTFEVIGHLETMIDALASCHMNYGTSVVVGVPPSAKMLTYDPMLLFTGRTWKGCVFGGLKSRDDVPKLVTEFLAKKFDLDQLITHVLPFKKISEGFELLNSGQSIRTVLTFHHHHHHHH

分子量

Approximately 44.0 kDa
纯度

Greater than 80% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)
参考文献

ADH7 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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