AGO2/Argonaute-2 蛋白, Human (sf9, His)

The AGO2/Argonaute-2 protein is critical for RNA-mediated silencing complex (RISC)-mediated gene silencing.In "minimal RISC," AGO2 binds to short guide RNAs, such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), to guide RISC for silencing with complementary mRNAs.AGO2/Argonaute-2 Protein, Human (sf9, His) is the recombinant human-derived AGO2/Argonaute-2 protein, expressed by Sf9 insect cells , with N-His, N-10*His labeled tag.

AGO2 是 RNA 介导的基因沉默 (RNAi) 中的关键参与者，由 RNA 诱导的沉默复合物 (RISC) 协调，对于多种调控过程是不可或缺的。“最小 RISC”配置涉及 AGO2 与短向导 RNA（例如 microRNA (miRNA) 或短干扰 RNA (siRNA)）连接，指导 RISC 靶向 mRNA 进行沉默。沉默机制取决于指导RNA与其靶mRNA之间的互补性。完美互补促进 AGO2 介导的核酸内切切割，而部分互补则独立于核酸内切酶活性抑制翻译。AGO2 可以通过与 7-甲基鸟苷帽结合，阻碍 eIF4-E 募集，或通过与 EIF6 相互作用，阻止核糖体亚基的结合来阻碍翻译起始。这种翻译抑制导致 mRNA 在细胞质加工体（P 体）中积累，最终可能导致 mRNA 降解。值得注意的是，AGO2 通过在不同的生长条件下抑制和促进翻译而表现出多功能性。此外，AGO2 是转录基因沉默 (TGS) 不可或缺的一部分，其中短 RNA（例如反基因 RNA）指导互补启动子区域的抑制。在微生物感染的情况下，AGO2 与病毒 miRNA 样小 RNA（例如 Sars-CoV-2 感染期间的 CoV2-miR-O7a）相关，可能抑制特定 mRNA（例如 BATF2），以逃避干扰素反应。

Human

Sf9 insect cells

N-10*His

Q9UKV8 (M1-A859)

27161  [NCBI]

MYSGAGPALAPPAPPPPIQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA

Approximately 99 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH7.4, 10% Glycerol, 2 mM DTT or 0.5 mM TCEP. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.