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AGO3 蛋白, Human (sf9, His)

目录号: HY-P74422
产品使用指南

AGO3 蛋白通过与 microRNA (miRNA) 等短 RNA 结合并抑制互补 mRNA 的翻译,对于 RNA 介导的基因沉默 (RNAi) 至关重要。它参与稳定干细胞中 Alu 重复序列产生的小 RNA 衍生物 (siRNA) 和加工过的 RNA 聚合酶 III 转录的 DR2 视黄酸反应元件 (RARE)。AGO3 蛋白, Human (sf9, His) 是重组的 AGO3 蛋白,由 Sf9 insect cells 表达,带有 N-His 标签。AGO3 蛋白, Human (sf9, His) 全长 860 个氨基酸,分子量约为 ~90 kDa。

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描述

The AGO3 protein is critical for RNA-mediated gene silencing (RNAi) by binding to short RNAs such as microRNAs (miRNAs) and inhibiting the translation of complementary mRNAs. It is involved in stabilizing small RNA derivatives (siRNA) produced by Alu repeats and the DR2 retinoic acid response element (RARE) transcribed by processed RNA polymerase III in stem cells. AGO3 Protein, Human (sf9, His) is the recombinant human-derived AGO3 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of AGO3 Protein, Human (sf9, His) is 860 a.a., with molecular weight of ~90 kDa.

研究背景

AGO3 是 RNA 介导的基因沉默 (RNAi) 中不可或缺的角色,通过与短 RNA(包括 microRNA (miRNA))结合并有效抑制带有互补序列的 mRNA 的翻译来发挥其调节作用。它的参与延伸到小RNA衍生物的稳定,特别是小干扰RNA (siRNA),源自经过加工的RNA聚合酶III转录的Alu重复序列,这些重复序列在干细胞中含有DR2视黄酸反应元件(RARE)。AGO3 被提议通过招募 mRNA 脱帽复合物(其中包括 EDC4)来协调 RNA 聚合酶 II 转录的编码 mRNA 特定子集随后的 siRNA 依赖性降解。在具有 RNA 切片活性的同时,AGO3 选择性地表现出这种切割功能,靶向引导靶互补区域内具有不同 5' 和 3' 侧翼序列的 RNA。

种属

Human

表达系统

Sf9 insect cells

标签

N-His

蛋白编号

Q9H9G7 (M1-A860)

基因 ID
蛋白结构
N-term
His
AGO3 (M1-A860)
Accession # Q9H9G7
C-term
同用名
Protein argonaute-3; hAgo3; eIF-2C 3; Argonaute-3; EIF2C3
分子量

Approximately 90 kDa

纯度

Greater than 88% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

AGO3 Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
AGO3 Protein, Human (sf9, His)
目录号:
HY-P74422
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