AGRP 蛋白, Human (HEK293, His)

AGRP proteins critically regulate body weight homeostasis and feeding behavior through the central melanocortin system. As an α-melanocyte-stimulating hormone antagonist, AGRP inhibits cAMP production and antagonizes stimulation of melanocortin receptors. AGRP Protein, Human (HEK293, His) is the recombinant human-derived AGRP protein, expressed by HEK293 , with C-His labeled tag.

AGRP 蛋白在体重稳态中发挥着至关重要的作用，并且是通过中枢黑皮质素系统调节进食行为的组成部分。作为一种 α 黑素细胞刺激激素拮抗剂，AGRP 抑制通过刺激下丘脑和肾上腺内黑皮质素受体介导的 cAMP 产生。虽然 AGRP 对 MC5R 表现出最小的活性，但 AGRP 作为 MC3R 和 MC4R 的反向激动剂，抑制它们的组成型活性。此外，AGRP 以抑制蛋白依赖性方式促进 MC3R 和 MC4R 的内吞作用，强调其在调节黑皮质素受体活性中的复杂参与。AGRP 与 MC3R、MC4R 和 MC5R 的相互作用进一步凸显了其在黑皮质素信号通路中的调节作用，使其成为体重调节和摄食控制的复杂平衡中的关键参与者。

Measured by its ability to antagonize alpha-MSH-induced cAMP accumulation in HEK293 human embryonic kidney cells transfected with human Melanocortin-4 Receptor. The ED₅₀ for this effect is typically ≤0.1019 µg/mL, corresponding to a specific activity is ≥9813.543 U/mg, in the presence of 10 ng/mL of alpha-MSH(HY-P0252).

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  Measured by its ability to antagonize alpha-MSH-induced cAMP accumulation in HEK293 human embryonic kidney cells transfected with human Melanocortin-4 Receptor. The ED₅₀ for this effect is typically 0.1019 µg/mL，corresponding to a specific activity is 9813.543 U/mg，in the presence of 10 ng/mL of alpha -MSH.

Human

HEK293

C-His

O00253 (A21-T132)

181  [NCBI]

AQMGLAPMEGIRRPDQALLPELPGLGLRAPLKKTTAEQAEEDLLQEAQALAEVLDLQDREPRSSRRCVRLHESCLGQQVPCCDPCATCYCRFFNAFCYCRKLGTAMNPCSRT

Approximately 16-21 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.2 or PBS, pH 7.2, 5% trehalose, 5% mannitol and 0.01% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.