AIF1 蛋白, Mouse (His)

AIF1 Protein, Mouse (His) is an actin-polymerizing and Rac1-activating protein that promotes vascular smooth muscle cell migration.

同种异体移植物炎症因子-1 (AIF-1)，也称为离子化钙结合接头分子 1 (Iba1)，是一种具有 EF 手钙结合结构域的细胞质蛋白，首次在大鼠心脏同种异体移植物的巨噬细胞中观察到在慢性排斥反应下。AIF-1 在单核细胞谱系中高表达，包括巨噬细胞和小胶质细胞，并通过属于 GTPases Rho 家族的 Rac1 参与吞噬作用和膜皱褶的形成。AIF-1 不存在于培养的人 VSMC 中，但由细胞因子诱导，AIF-1 的过度表达会导致 VSMC 生长和细胞周期基因表达增加^{[1][2]< /sup>。}

Mouse

E. coli

N-6*His

O70200 (S2-P147)

11629  [NCBI]

SQSRDLQGGKAFGLLKAQQEERLEGINKQFLDDPKYSNDEDLPSKLEAFKVKYMEFDLNGNGDIDIMSLKRMLEKLGVPKTHLELKRLIREVSSGSEETFSYSDFLRMMLGKRSAILRMILMYEEKNKEHKRPTGPPAKKAISELP

Approximately 24 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.

• [1]. Autieri MV, et, al. AIF-1 is an actin-polymerizing and Rac1-activating protein that promotes vascular smooth muscle cell migration. Circ Res. 2003 May 30;92(10):1107-14.  [Content Brief]

  [2]. Kishikawa S, et, al. Allograft inflammatory factor 1 is a regulator of transcytosis in M cells. Nat Commun. 2017 Feb 22;8:14509.  [Content Brief]