ALCAM/CD166 蛋白, Human (Biotinylated, HEK293, His)

The ALCAM/CD166 protein is a cell adhesion molecule that mediates heterotypic and homotypic cell-to-cell contacts, promoting T cell activation, immune synapse formation, and LILRB4-mediated inhibition of T cell proliferation. ALCAM/CD166 Protein, Human (Biotinylated, HEK293, His) is the recombinant human-derived ALCAM/CD166 protein, expressed by HEK293 , with C-His labeled tag. The total length of ALCAM/CD166 Protein, Human (Biotinylated, HEK293, His) is 499 a.a., with molecular weight of 70-80 kDa.

ALCAM/CD166 蛋白作为关键的细胞粘附分子，协调一系列细胞相互作用。它通过与 CD6 相互作用介导异型细胞间接触和同型细胞间接触，促进 T 细胞活化和增殖。ALCAM 通过与 CD6 结合，对免疫突触的形成和成熟做出重大贡献。此外，它还促进与表达 ALCAM 的细胞的同型相互作用。ALCAM 作为 LILRB4 受体的配体，增强 LILRB4 介导的 T 细胞增殖抑制。该蛋白质对于正常造血干细胞在骨髓中的植入是必不可少的，并通过同型相互作用介导树突细胞附着到内皮细胞上。ALCAM 在抑制内皮细胞迁移、促进内皮管形成方面发挥着至关重要的作用。它对于淋巴管网络和造血的组织至关重要，确保骨髓中造血干细胞的正常数量。ALCAM 的多种功能包括促进体外成骨细胞增殖和分化，以及影响神经突延伸、轴突生长和轴突导向。值得注意的是，它通过竞争相同的相互作用伙伴来抑制膜结合亚型，通过同型相互作用限制细胞附着，并同时促进内皮细胞迁移，同时抑制内皮细胞管形成。

Measured by its binding ability in a functional ELISA. When immobilized Anti-ALCAM Antibody hFc at 2 μg/mL (100μL/Well), can bind Biotinylated Human ALCAM-His Tag and the EC₅₀ is <5 μg/mL.

Human

HEK293

C-His

Q13740 (W28-A526)

214  [NCBI]

70-80 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.