2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3) Phosphatase
  5. Alkaline Phosphatase
  6. ALPI 蛋白, Human (HEK293, Fc)

ALPI 蛋白, Human (HEK293, Fc)

目录号: HY-P72821
产品使用指南

ALPI 蛋白质是一种碱性磷酸酶，可有效水解多种磷酸盐化合物。ALPI 蛋白, Human (HEK293, Fc) 是重组的 ALPI 蛋白，由 HEK293 表达，带有 C-hFc 标签。ALPI 蛋白, Human (HEK293, Fc) 全长 503 个氨基酸，分子量为 90-95 kDa。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

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描述

ALPI Proteinas, an alkaline phosphatase, effectively hydrolyzes diverse phosphate compounds. ALPI Protein, Human (HEK293, Fc) is the recombinant human-derived ALPI protein, expressed by HEK293 , with C-hFc labeled tag. The total length of ALPI Protein, Human (HEK293, Fc) is 503 a.a., with molecular weight of 90-95 kDa.
研究背景

ALPI 蛋白是一种碱性磷酸酶，能够水解各种磷酸盐化合物。
种属

Human
表达系统

HEK293
标签

C-hFc
蛋白编号

P09923 (M1-D503)
基因 ID

248  [NCBI]
蛋白结构
N-term
ALPI (M1-D503)
Accession # P09923
hFc
C-term
同用名
Intestinal-type alkaline phosphatase; IAP; ALPI; Alkaline Phosphatase
氨基酸序列

MQGPWVLLLLGLRLQLSLGVIPAEEENPAFWNRQAAEALDAAKKLQPIQKVAKNLILFLGDGLGVPTVTATRILKGQKNGKLGPETPLAMDRFPYLALSKTYNVDRQVPDSAATATAYLCGVKANFQTIGLSAAARFNQCNTTRGNEVISVMNRAKQAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADMPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPADASQNGIRLDGKNLVQEWLAKHQGAWYVWNRTELMQASLDQSVTHLMGLFEPGDTKYEIHRDPTLDPSLMEMTEAALRLLSRNPRGFYLFVEGGRIDHGHHEGVAYQALTEAVMFDDAIERAGQLTSEEDTLTLVTADHSHVFSFGGYTLRGSSIFGLAPSKAQDSKAYTSILYGNGPGYVFNSGVRPDVNESESGSPDYQQQAAVPLSSETHGGEDVAVFARGPQAHLVHGVQEQSFVAHVMAFAACLEPYTACDLAPPACTTD

分子量

90-95 kDa
纯度

Greater than 83% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

ALPI Protein, Human (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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