1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) Phosphatase
  4. Alkaline Phosphatase
  5. ALPI 蛋白, Human (His)

ALPI 蛋白, Human (His)

目录号: HY-P71692
产品使用指南

ALPI 蛋白质是一种碱性磷酸酶,可有效水解多种磷酸盐化合物。ALPI 蛋白, Human (His) 是重组的 ALPI 蛋白,由 E. coli 表达,带有 N-His 标签。ALPI 蛋白, Human (His) 全长 475 个氨基酸,分子量约为 ~55.5 kDa。

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     可免费申领三个不同产品的试用装。

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规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

ALPI Proteinas, an alkaline phosphatase, effectively hydrolyzes diverse phosphate compounds. ALPI Protein, Human (His) is the recombinant human-derived ALPI protein, expressed by E. coli , with N-His labeled tag. The total length of ALPI Protein, Human (His) is 475 a.a., with molecular weight of ~55.5 kDa.

研究背景

ALPI 蛋白是一种碱性磷酸酶,能够水解各种磷酸盐化合物。

种属

Human

表达系统

E. coli

标签

N-His

蛋白编号

P09923 (26E-500C)

基因 ID

248  [NCBI]

蛋白结构
N-term
His
ALPI (26E-500C)
Accession # P09923
C-term
同用名
Alkaline phosphatase intestinal; Glycerophosphatase; IAP
氨基酸序列

ENPAFWNRQAAEALDAAKKLQPIQKVAKNLILFLGDGLGVPTVTATRILKGQKNGKLGPETPLAMDRFPYLALSKTYNVDRQVPDSAATATAYLCGVKANFQTIGLSAAARFNQCNTTRGNEVISVMNRAKQAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADMPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPADASQNGIRLDGKNLVQEWLAKHQGAWYVWNRTELMQASLDQSVTHLMGLFEPGDTKYEIHRDPTLDPSLMEMTEAALRLLSRNPRGFYLFVEGGRIDHGHHEGVAYQALTEAVMFDDAIERAGQLTSEEDTLTLVTADHSHVFSFGGYTLRGSSIFGLAPSKAQDSKAYTSILYGNGPGYVFNSGVRPDVNESESGSPDYQQQAAVPLSSETHGGEDVAVFARGPQAHLVHGVQEQSFVAHVMAFAACLEPYTACDLAPPAC

分子量

Approximately 55.5 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ALPI Protein, Human (His)
目录号:
HY-P71692
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