1. 重组蛋白
  2. CD Antigens Enzymes & Regulators
  3. Epithelial cell CD Proteins Endothelial cell CD Proteins Hydrolases (EC 3)
  4. Glutamyl Aminopeptidase/CD249
  5. Aminopeptidase A 蛋白, Rat (sf9, His)

Aminopeptidase A 蛋白, Rat (sf9, His)

目录号: HY-P72826
COA 产品使用指南

氨基肽酶 A 受钙调节,通过优先裂解肽(包括血管紧张素 II)的 N 末端酸性残基来调节中枢性高血压。Aminopeptidase A 蛋白, Rat (sf9, His) 是重组的 氨基肽酶 A 蛋白,由 Sf9 insect cells 表达,带有 N-His 标签。Aminopeptidase A 蛋白, Rat (sf9, His) 全长 905 个氨基酸,分子量约为 ~116 kDa。

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规格 价格 是否有货 数量
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5 μg ¥1150 In-stock
10 μg ¥1900 In-stock
50 μg ¥5350 In-stock
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Aminopeptidase A Protein, Rat (sf9, His) 相关产品:

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Aminopeptidase A, modulated by calcium, regulates central hypertension by preferentially cleaving N-terminal acidic residues from peptides, including angiotensin II. Aminopeptidase A Protein, Rat (sf9, His) is the recombinant rat-derived Aminopeptidase A protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of Aminopeptidase A Protein, Rat (sf9, His) is 905 a.a., with molecular weight of ~116 kDa.

研究背景

氨基肽酶 A 是一种关键的调节酶,通过表现出钙调节的特异性从肽中裂解 N 末端酸性残基来控制中枢性高血压,在处理血管紧张素 II 方面具有显着的意义。通过这种催化活性,氨基肽酶 A 有助于与血压调节相关的生理过程的复杂调节,强调了其在中枢性高血压途径中的关键作用。

生物活性

Measured by its ability to cleave 200 μM substrate Glu-7-amido-4-methylcoumarin that incubate at 37 ℃ in kinetic mode for 5 minutes. The specific activity is 25877.184 pmol/min/µg. 

种属

Rat

表达系统

Sf9 insect cells

标签

N-His

蛋白编号

P50123-1 (R41-P945)

基因 ID
蛋白结构
N-term
His
ENPEP (R41-P945)
Accession # P50123-1
C-term
同用名
Glutamyl aminopeptidase; EAP; AP-A; CD249; ENPEP
氨基酸序列

RSCEPGTTPAPSNPPPHTSTALPPQDQNVCPDSDDESGEWKNFRLPDFIQPVHYDLEVKVLMEEDRYTGIVSISVNLSKDTRDLWLHIRETRITKLPELRRPSGEQVPIRRCFEYKKQEYVVIQAEEDLAATSGDSVYRLTIEFEGWLNGSLVGFYRTTYTEDGQTKSIAATDHEPTDARKSFPCFDEPNKKATYNISLIHPKEYSALSNMPVEKKETLDNDWKKTTFMKSVPMSTYLVCFAVHQFTSIQRTSRSGKPLTVYVQPNQKQTAEYAANITKAVFDFFEDYFAMEYSLPKLDKIAIPDFGTGAMENWGLVTYRETNLLYDPLLSASSNQQRVASVVAHELVHQWFGNIVTMDWWDDLWLNEGFASFFEFLGVNHAEADWQMLSQVLLEDVLPVQEDDSLMSSHPVVVTVSTPAEITSVFDGISYSKGASILRMLQDWITPEKFQKGCQIYLENFKFKNAKTSDFWDSLEKASNQPVKEVMDTWTSQMGYPVVTVSGKQNVTQKRFLLDYKADPSQPPSALGYTWNIPIKWTENGNSNITVYYRSNREGITLNANLSGDGFLKINPDHIGFYRVNYEAETWDWIAETLSSNHMNFSSADRSSFIDDAFALARAQLLDYEKALNLTRYLTSEKDFLPWERVISAVSYIISMFEDDRELYPLIETYFRSQVKPIADSLGWQDTGSHITKLLRASVLGFACKMGAGEALGNASQLFEAWLKGNESIPVNLRLLVYRYGMQNSGNEAAWNYTLEQYQKTSLAQEKEKLLYGLASVKDVTLLARYLEMLKDPNIIKTQDVFTVIRYISYNSYGKSMAWNWIQLNWDYLVNRFTINDRYLGRIVTIAEPFNTELQLWQMQSFFAKYPNAGAGAKPREQVLETVKNNIEWLKLNRKSISEWFTSMP

分子量

Approximately 116 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Aminopeptidase A Protein, Rat (sf9, His)
目录号:
HY-P72826
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