Amphiregulin 蛋白, Mouse (HEK293, Fc)

Amphiregulin Protein, an EGFR ligand, acts as an autocrine growth factor and mitogen for diverse cells, including astrocytes, Schwann cells, and fibroblasts. Engaging with CNIH in its immature stage, Amphiregulin's EGFR activation emphasizes its vital role in regulating cell growth and orchestrating various cellular functions across different cell types. Amphiregulin Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Amphiregulin protein, expressed by HEK293 , with N-hFc labeled tag. The total length of Amphiregulin Protein, Mouse (HEK293, Fc) is 149 a.a., with molecular weight of 45-50 kDa.

双调蛋白是 EGF 受体 (EGFR) 的配体，既可作为自分泌生长因子，又可作为多种靶细胞（如星形胶质细胞、雪旺细胞和成纤维细胞）的有丝分裂原。它的影响跨越细胞增殖，并且在其不成熟的前体阶段，双调蛋白与 CNIH 相互作用。这种多功能配体激活 EGFR 的能力强调了其在调节细胞生长中的关键作用，并强调了其在协调不同细胞类型的各种细胞功能中的重要性。

Measured in a cell proliferation assay using Balb/3T3 cells. The ED₅₀ for this effect is 19.54 ng/mL, corresponding to a specific activity is 5.11×10⁴ units/mg.

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  Measured in a cell proliferation assay using Balb/3T3 cells. The ED₅₀ for this effect is 19.54 ng/mL, corresponding to a specific activity is 5.11×10⁴ units/mg.

Mouse

HEK293

N-hFc

P31955 (V100-A248)

11839  [NCBI]

VIKPKKNKTEGEKSTEKPKRKKKGGKNGKGRRNKKKKNPCTAKFQNFCIHGECRYIENLEVVTCNCHQDYFGERCGEKSMKTHSEDDKDLSKIAVVAVTIFVSAIILAAIGIGIVITVHLWKRYFREYEGETEERRRLRQENGTVHAIA

45-50 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.