Animal-Free EGF 蛋白, Mouse (His)

EGF Protein, a growth factor, is essential for cell growth, proliferation, and differentiation. It binds to the EGF receptor, activating signaling pathways that regulate cell survival and tissue repair. EGF Protein has therapeutic potential in promoting wound healing, tissue regeneration, and cancer treatment. Its role in cellular processes makes it a subject of interest in biomedical research. Animal-Free EGF Protein, Mouse (His) is the recombinant mouse-derived animal-FreeEGF protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free EGF Protein, Mouse (His) is 53 a.a., with molecular weight of ~6.98 kDa.

EGF是一种有效的生长因子，可促进体内和体外各种表皮和上皮组织以及细胞培养物中某些成纤维细胞的增殖。它通过激活 EGFR 和镁通道 TRPM6 来刺激肾远曲小管中镁的重吸收，从而起到促镁激素的作用。EGF 还与 EGFR 相互作用，促进 EGFR 二聚化，并与 RHBDF1 和 RHBDF2 形成相互作用，可能调节其细胞内定位和降解。

Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <80 pg/mL. The specific activity of recombinant mouse EGF is approximately >1x10⁶ IU/mg.

Mouse

E. coli

C-His

P01132 (N977-R1029)

13645  [NCBI]

MNSYPGCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELR

Approximately 6.98 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.