Animal-Free GCP-2/CXCL6 蛋白, Human (His)

The GCP-2/CXCL6 protein is a chemokine that eliminates pathogens by attracting neutrophils, basophils, and T cells and is an important mediator in inflammation. It activates neutrophils by binding to the G protein-coupled receptors CXCR1 and CXCR2, which are mainly expressed on neutrophils, monocytes, and endothelial cells. Animal-Free GCP-2/CXCL6 Protein, Human (His) is the recombinant human-derived animal-FreeGCP-2/CXCL6 protein, expressed by E. coli , with N-His labeled tag. The total length of Animal-Free GCP-2/CXCL6 Protein, Human (His) is 75 a.a., with molecular weight of ~8.97 kDa.

CXCL8 是一种趋化因子，通过吸引中性粒细胞、嗜碱性粒细胞和 T 细胞来消除病原体并保护宿主免受感染，从而充当炎症反应的关键介质。这种蛋白质在激活中性粒细胞方面也起着至关重要的作用。CXCL8 因炎症刺激而释放后，通过与 G 蛋白偶联受体 CXCR1 和 CXCR2（主要在中性粒细胞、单核细胞和内皮细胞中表达）结合来发挥作用。这种结合触发 CXCR1/CXCR2 受体释放 G 蛋白异三聚体（α、β、γ 亚基），从而激活下游信号通路，包括 PI3K 和 MAPK 通路。CXCL8 的同二聚体结构有利于其相互作用，并且已被证明可以与 TNFAIP6 相互作用，破坏趋化因子与糖胺聚糖的结合。这种多方面的作用强调了 CXCL8 在炎症期间协调免疫反应的重要性。

Measure by its ability to chemoattract BaF3 cells transfected with human CXCR2. The ED₅₀ for this effect is <10 ng/mL.

Human

E. coli

N-His

P80162 (V40-N114)

6372  [NCBI]

VSAVLTELRCTCLRVTLRVNPKTIGKLQVFPAGPQCSKVEVVASLKNGKQVCLDPEAPFLKKVIQKILDSGNKKN

Approximately 8.97 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.