Animal-Free IFN-lambda 2/IL-28A 蛋白, Human (His)

IFN-lambda 2/IL-28A protein is a multifunctional cytokine with antiviral, antitumor, and immunomodulatory activities. It plays a key role in antiviral host defense, particularly within epithelial tissues. Animal-Free IFN-lambda 2/IL-28A Protein, Human (His) is the recombinant human-derived animal-FreeIFN-lambda 2/IL-28A protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IFN-lambda 2/IL-28A Protein, Human (His) is 174 a.a., with molecular weight of ~20.60 kDa.

IFN-lambda 2/IL-28A 蛋白是一种多功能细胞因子，具有抗病毒、抗肿瘤和免疫调节活性。它在抗病毒宿主防御中发挥着关键作用，特别是在上皮组织内。作为包含 IL10RB 和 IFNLR1 的异二聚体 II 类细胞因子受体的配体，其参与激活 JAK/STAT 信号通路，导致 IFN 刺激基因 (ISG) 的表达，从而建立抗病毒状态。值得注意的是，其受体分布受到限制，由于受体 IFNLR1 的细胞类型选择性表达，其主要在上皮细胞中发挥作用。虽然不被认为对于阴道感染中病毒激活的早期宿主防御至关重要，但事实证明它对于 Toll 样受体 (TLR) 诱导的抗病毒防御至关重要。它在肠上皮内的抗病毒免疫防御中发挥重要作用。此外，IFN-lambda 2/IL-28A 通过上调 MHC I 类抗原表达来促进免疫调节，增强其对免疫反应的影响。

Measure by its ability to protect HepG2 cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is <20 ng/mL.

Human

E. coli

C-His

Q8IZJ0 (P27-V200)

282616  [NCBI]

MPVARLHGALPDARGCHIAQFKSLSPQELQAFKRAKDALEESLLLKDCRCHSRLFPRTWDLRQLQVRERPMALEAELALTLKVLEATADTDPALVDVLDQPLHTLHHILSQFRACIQPQPTAGPRTRGRLHHWLYRLQEAPKKESPGCLEASVTFNLFRLLTRDLNCVASGDLCV

Approximately 20.60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.