Animal-Free IL-10 蛋白, Pig (His)

IL-10 Protein, a key immune regulator, mitigates inflammation by binding to its receptor (IL10RA/IL10RB), activating JAK1 and STAT2, leading to STAT3-mediated anti-inflammatory gene expression. IL-10 targets APCs, reducing pro-inflammatory cytokines and hindering antigen presentation. It controls macrophage inflammation through metabolic reprogramming. Existing as a homodimer, IL-10 interacts with IL10RA/IL10RB. Animal-Free IL-10 Protein, Pig (His) is the recombinant pig-derived animal-FreeIL-10 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-10 Protein, Pig (His) is 157 a.a., with molecular weight of ~19.1 kDa.

IL-10是一种主要的免疫调节细胞因子，通过发挥深远的抗炎功能，有效限制炎症引起的过度组织破坏，在调节免疫系统中发挥着关键作用。从机制上讲，IL-10 与其异四聚体受体（由 IL10RA 和 IL10RB 组成）结合，启动 JAK1 和 STAT2 介导的 STAT3 磷酸化。随后，磷酸化的 STAT3 易位至细胞核，驱动抗炎介质的表达。IL-10 特异性靶向抗原呈递细胞 (APC)，例如巨噬细胞和单核细胞，抑制其释放促炎细胞因子，包括 GM-CSF、G-CSF、IL-1 α、IL-1 β、IL-6 、IL-8 和 TNF-α。此外，IL-10 通过减少 MHC II 类和共刺激分子的表达来干扰抗原呈递，从而阻碍它们诱导 T 细胞激活的能力。此外，IL-10 通过重新编程重要的代谢途径（包括 mTOR 信号传导）来维持对巨噬细胞炎症反应的控制。在结构上，IL-10 形成同二聚体，并在其调节功能中与 IL10RA 和 IL10RB 结合。

Pig

E. coli

C-His

Q29055 (S19-N175)

397106  [NCBI]

MSIKSENSCIHFPTSLPHMLRELRAAFGPVKSFFQTKDQMGDLLLTGSLLEDFKGYLGCQALSEMIQFYLEDVMPKAESDGEDIKEHVNSLGEKLKTLRLRLRRCHQFLPCENKSKAVEEVKSAFSKLQERGVYKAMGEFDIFINYIEAYMTMKMRKN

Approximately 19.1 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<0.01 EU per 1 μg of the protein by the LAL method.