Animal-Free IL-12 alpha 蛋白, Mouse (His)

IL-12 alpha protein forms two cytokines: IL-12 and IL-35. It modulates T cell and natural killer cell responses, induces IFN-γ production, and promotes Th1 cell differentiation. Animal-Free IL-12 alpha Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIL-12 alpha protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-12 alpha Protein, Mouse (His) is 193 a.a., with molecular weight of ~22.65 kDa.

IL-12α蛋白能够与IL12B异二聚形成IL-12细胞因子或与EBI3/IL27B异二聚形成IL-35细胞因子。IL-12 主要由专业抗原呈递细胞产生，例如 B 细胞、树突状细胞、巨噬细胞和粒细胞。它在调节 T 细胞和自然杀伤细胞反应、诱导干扰素 γ (IFN-gamma) 产生、促进辅助性 T 1 (Th1) 细胞分化以及桥接先天抵抗与适应性免疫方面发挥着至关重要的作用。IL-12 受体由 IL12R1 和 IL12R2 亚基组成，通过启动细胞底物（包括 TYK2 和 JAK2 激酶）的酪氨酸磷酸化来介导 IL-12 的生物效应。招募的磷酸化 STAT4 易位到细胞核，在那里调节细胞因子和生长因子反应基因的表达。作为 IL-35 的一部分，IL-12 α 蛋白在维持肝脏微环境中的免疫稳态中发挥着重要作用，并充当免疫抑制细胞因子。信号传导通过由 IL12RB2 和 gp130/IL6ST 异二聚体或同二聚体组成的非常规受体发生，需要转录因子 STAT1 和 STAT4 的参与。STAT1 和 STAT4 之间形成独特的异二聚体，能够与特定的 DNA 位点结合。IL-12α蛋白可与IL12B形成二硫键连接的异二聚体，称为白细胞介素IL-12，以及与EBI3/IL27B形成非二硫键连接的异二聚体，称为白细胞介素IL-35。此外，它与 NBR1 相互作用，促进 IL-12 分泌。

Measure by its ability to induce proliferation in T-cell enriched PBMC. The ED₅₀ for this effect is <0.2 ng/mL.

Mouse

E. coli

C-His

P43431 (R23-A215)

16159  [NCBI]

MRVIPVSGPARCLSQSRNLLKTTDDMVKTAREKLKHYSCTAEDIDHEDITRDQTSTLKTCLPLELHKNESCLATRETSSTTRGSCLPPQKTSLMMTLCLGSIYEDLKMYQTEFQAINAALQNHNHQQIILDKGMLVAIDELMQSLNHNGETLRQKPPVGEADPYRVKMKLCILLHAFSTRVVTINRVMGYLSSA

Approximately 22.65 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.