Animal-Free IL-21 蛋白, Mouse (His)

IL-21 is a cytokine with immunomodulatory capabilities that orchestrates the transition from innate to adaptive immunity. It induces the production of IgG(1) and IgG(3) by B cells, thereby forming a humoral immune response. Animal-Free IL-21 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIL-21 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-21 Protein, Mouse (His) is 129 a.a., with molecular weight of ~15.9 kDa.

IL-21 是一种具有免疫调节能力的细胞因子，在协调先天免疫和适应性免疫之间的微妙转变中发挥着关键作用。这种多方面的调节剂在诱导 B 细胞中产生 IgG(1) 和 IgG(3) 方面发挥了作用，从而证实了其在形成体液免疫反应中的关键作用。IL-21 在促进滤泡辅助 T (Tfh) 细胞的产生和永久存在以及生发中心的复杂形成方面发挥着重要作用。它与 IL6 协同作用，对 Tfh 细胞的早期发育发挥指挥作用，为急性病毒感染期间的强大抗体反应做出不可或缺的贡献。除了 B 细胞之外，IL-21 将其影响扩展到自然杀伤 (NK) 细胞领域，与 IL15 协同作用，促进其增殖和成熟。此外，IL-21 还可以发挥其调节能力，引导成熟 B 细胞和 T 细胞响应激活刺激而增殖和成熟。它与 IL15 和 IL18 协同作用，协调 T 细胞和 NK 细胞中干扰素 γ 的产生。值得注意的是，在 T 细胞介导的免疫反应过程中，IL-21 作为潜在的抑制剂介入，抑制树突状细胞的激活和成熟，揭示了免疫动力学中微妙的调节作用。

Measure by its ability to enhance IFN gamma secretion in NK-92 cells. The ED₅₀ for this effect is <6 ng/mL. The specific activity of recombinant mouse IL-21 is >1.6 x 10⁵IU/mg

Mouse

E. coli

C-His

Q9ES17 (H18-S146)

60505  [NCBI]

MHKSSPQGPDRLLIRLRHLIDIVEQLKIYENDLDPELLSAPQDVKGHCEHAAFACFQKAKLKPSNPGNNKTFIIDLVAQLRRRLPARRGGKKQKHIAKCPSCDSYEKRTPKEFLERLKWLLQKMIHQHLS

Approximately 15.9 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.