Animal-Free IL-9 蛋白, Mouse (His)

IL-9 is secreted by T helper 2 lymphocytes, mast cells, and NKT cells and plays an important role in antiparasitic immune responses, affecting intestinal permeability, adaptive immunity, and T cell subset differentiation.It promotes TH17 cell and mast cell proliferation through IL9R and IL2RG receptor activation, triggering JAK1 and JAK3 kinases and subsequent STAT-mediated transcription.Animal-Free IL-9 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIL-9 protein, expressed by E.coli , with N-His labeled tag.

IL-9 是一种多功能细胞因子，主要由 T 辅助细胞 2 淋巴细胞以及肥大细胞或 NKT 细胞分泌，在针对寄生虫的免疫反应中发挥着至关重要的作用。除了抗寄生虫功能外，IL-9 还影响肠上皮通透性和适应性免疫。它在诱导特定 T 细胞亚群（例如产生 IL-17 的辅助 T 细胞（TH17））的分化中发挥着关键作用，并促进肥大细胞的增殖和分化。从机制上讲，IL-9 通过由 IL9R 亚基和信号转导亚基 IL2RG 组成的受体发挥其多种生物学作用。刺激该受体会导致 JAK1 和 JAK3 激酶活性快速激活，启动 STAT1、STAT3 和 STAT5 介导的转录程序。分化基因的诱导似乎仅由 STAT1 介导，而细胞免于凋亡的保护则取决于 STAT3 和 STAT5 的协同作用。IL-9 直接与 IL9R 和 IL2RG 相互作用，协调复杂的相互作用网络来调节免疫反应和细胞过程。

Measure by its ability to induce proliferation in MO7e cells.The ED₅₀ for this effect is<0.2 ng/mL.The specific activity of recombinant mouse IL9 is >5 x10⁶ IU/mg.

Mouse

E. coli

N-His

P15247 (Q19-P144)

16198  [NCBI]

QRCSTTWGIRDTNYLIENLKDDPPSKCSCSGNVTSCLCLSVPTDDCTTPCYREGLLQLTNATQKSRLLPVFHRVKRIVEVLKNITCPSFSCEKPCNQTMAGNTLSFLKSLLGTFQKTEMQRQKSRP

Approximately 14.97 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.