Animal-Free Pleiotrophin 蛋白, Human (His)

Pleiotrophin is a secreted growth factor that signals through cell surface proteoglycan and non-proteoglycan receptors to influence a variety of cellular processes. It binds to chondroitin sulfate (CS) groups, regulates proliferation, survival and differentiation, and inhibits long-term synaptic potentiation of neurons. Animal-Free Pleiotrophin Protein, Human (His) is the recombinant human-derived animal-FreePleiotrophin protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free Pleiotrophin Protein, Human (His) is 136 a.a., with molecular weight of ~19.89 kDa.

多效蛋白是一种分泌性生长因子，可通过细胞表面蛋白聚糖和非蛋白聚糖受体转导其信号。它与细胞表面蛋白聚糖受体的硫酸软骨素 (CS) 基团结合，调节各种组织（包括神经元和骨骼）中的细胞增殖、存活、生长、分化和迁移等过程。多效蛋白还通过抑制长期突触增强，在突触可塑性和学习相关行为中发挥着至关重要的作用。通过与 PTPRZ1 结合，多效蛋白中和 CS 链的负电荷，诱导 PTPRZ1 聚集并使其磷酸酶活性失活，导致 PTPRZ1 底物（如 ALK、CTNNB1 或 AFAP1L2）的酪氨酸磷酸化增加，从而激活 PI3K-AKT 通路。它与 PTPRZ1 和整合素 α-V/β-3 形成复合物，刺激内皮细胞迁移。在成年海马体中，多效蛋白通过激活 AKT 信号通路，通过 ALK 促进新生颗粒神经元的树突分枝、脊柱发育和功能整合。此外，它还与 GPC2、SDC3 和其他受体相互作用，介导与骨形成、神经干细胞增殖和分化、造血再生以及女性生殖系统和听觉反应中的各种生理过程相关的多种功能。复杂的相互作用网络强调了多效蛋白在细胞和组织水平调节机制中的多方面作用。

Human

E. coli

C-His

P21246 (G33-D168)

5764  [NCBI]

MGKKEKPEKKVKKSDCGEWQWSVCVPTSGDCGLGTREGTRTGAECKQTMKTQRCKIPCNWKKQFGAECKYQFQAWGECDLNTALKTRTGSLKRALHNAECQKTVTISKPCGKLTKPKPQAESKKKKKEGKKQEKMLD

Approximately 19.89 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.