Animal-Free TGF beta 1/TGFB1 蛋白, Mouse (His)

In its proprotein form, the TGF beta-1/TGFB1 protein serves as a precursor to latency-associated peptide (LAP) and active transforming growth factor beta-1 (TGF-beta-1) chains.Critical to maintaining the latent state of TGF-β-1 within the extracellular matrix, preprotein interacts with “environmental molecules” such as LTBP1, LRRC32/GARP, and LRRC33/NRROS to regulate TGF-β-1 activation.Animal-Free TGF beta 1/TGFB1 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeTGF beta 1/TGFB1 protein, expressed by E.coli , with C-His labeled tag.

TGF beta-1 (TGFB1) 蛋白以其原蛋白形式充当潜伏相关肽 (LAP) 和活性转化生长因子 beta-1 (TGF-beta-1) 链的前体。该蛋白原对于在细胞外基质内储存期间维持 TGF-β-1 链处于潜在状态至关重要。与各种“环境分子”（包括 LTBP1、LRRC32/GARP 和 LRRC33/NRROS）的相互作用在调节 TGF-β-1 的激活中发挥着关键作用。具体而言，LRRC33/NRROS 参与巨噬细胞和小胶质细胞中 TGF-β-1 的激活，而 LRRC32/GARP 控制其在激活的调节性 T 细胞 (Treg) 表面的激活。此外，前蛋白与整合素（ITGAV:ITGB6 或 ITGAV:ITGB8）相互作用，导致潜伏期相关肽链扭曲并随后释放活性 TGF-β-1。

Measure by its ability to inhibit the IL-4 dependent proliferation in HT-2 cells.The ED₅₀ for this effect is<0.1 ng/mL.

Mouse

E. coli

C-His

P04202 (A279-S390)

21803  [NCBI]

MALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Approximately 13.8 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in 10 mM HCl. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.