Animal-Free TNF-beta/TNFSF1 蛋白, Human (His)

TNF-β protein acts as a homotrimeric cytokine that binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR, and TNFRSF14/HVEM. Animal-Free TNF-beta/TNFSF1 Protein, Human (His) is the recombinant human-derived animal-FreeTNF-beta/TNFSF1 protein, expressed by E. coli , with N-His labeled tag. The total length of Animal-Free TNF-beta/TNFSF1 Protein, Human (His) is 171 a.a., with molecular weight of ~18.65 kDa.

多项研究表明，同源三聚体结构的 TNF-β 蛋白表现出与 TNFRSF1A/TNFR1、TNFRSF1B/TNFBR 和 TNFRSF14/HVEM 的结合能力。此外，在与 LTB 的异三聚体关联中，它与 TNFRSF3/LTBR 形成相互作用，强调了其不同的受体参与。这种细胞因子主要由淋巴细胞产生，在体外和体内均表现出针对多种肿瘤细胞的细胞毒性作用。在结构上，TNF-β以同源三聚体和异源三聚体形式存在，后者包含两个LTB和一个LTA亚基，或者在较小程度上包含两个LTA和一个LTB亚基。此外，TNF-β 与 TNFRSF14 相互作用，强调其在介导复杂免疫反应中的关键作用。

Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED₅₀ for this effect is <3 pg/mL. The specific activity of recombinant human TNF beta is > 3.3x10⁸ IU/mg.

Human

E. coli

N-His

P01374 (L35-L205)

4049  [NCBI]

LPGVGLTPSAAQTARQHPKMHLAHSTLKPAAHLIGDPSKQNSLLWRANTDRAFLQDGFSLSNNSLLVPTSGIYFVYSQVVFSGKAYSPKATSSPLYLAHEVQLFSSQYPFHVPLLSSQKMVYPGLQEPWLHSMYHGAAFQLTQGDQLSTHTDGIPHLVLSPSTVFFGAFAL

Approximately 18.65 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.