Annexin A2/ANXA2 蛋白, Mouse (His)

Annexin A2 (ANXA2) is a calcium-regulated membrane-binding protein with heightened calcium affinity in the presence of anionic phospholipids. Known for its potential involvement in the heat-stress response, ANXA2 operates as a heterotetramer, comprising two light chains of S100A10/p11 and two heavy chains of ANXA2/p36. It inhibits PCSK9-enhanced LDLR degradation, potentially through translational mechanisms, while also competing with LDLR for PCSK9 binding. ANXA2 interacts with various proteins, including ATP1B1, DYSF, COCH, and CEACAM1, with the latter interaction being regulated by CEACAM1 phosphorylation. Moreover, ANXA2 interacts with PCSK9, forming a complex that hinders LDLR degradation. Additionally, it associates with APPL2 and APPL1, facilitating the targeting of APPL2 to endosomes in parallel with RAB5A. The intricate network of ANXA2 interactions underscores its versatile role in cellular processes and regulatory pathways. Annexin A2/ANXA2 Protein, Mouse (His) is the recombinant mouse-derived Annexin A2/ANXA2 protein, expressed by E. coli , with N-His labeled tag. The total length of Annexin A2/ANXA2 Protein, Mouse (His) is 338 a.a., with molecular weight of ~39.6 kDa.

膜联蛋白 A2 (ANXA2) 是一种钙调节膜结合蛋白，在阴离子磷脂存在下具有增强的钙亲和力。 ANXA2 因其可能参与热应激反应而闻名，它作为异四聚体发挥作用，由 S100A10/p11 的两条轻链和 ANXA2/p36 的两条重链组成。它可能通过翻译机制抑制 PCSK9 增强的 LDLR 降解，同时还与 LDLR 竞争 PCSK9 结合。 ANXA2 与多种蛋白质相互作用，包括 ATP1B1、DYSF、COCH 和 CEACAM1，后者相互作用受 CEACAM1 磷酸化调节。此外，ANXA2 与 PCSK9 相互作用，形成阻碍 LDLR 降解的复合物。此外，它与 APPL2 和 APPL1 结合，促进 APPL2 与 RAB5A 并行靶向内体。 ANXA2 相互作用的复杂网络强调了其在细胞过程和调控途径中的多功能作用。

Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 0.1183 ng/mL, corresponding to a specific activity is 8.453×10⁶ units/mg.

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  Measured in a cell proliferation assay using A549cells. The ED₅₀ for this effect is 0.1183 ng/mL, corresponding to a specific activity is 8.453×10⁶ units/mg.

Mouse

E. coli

N-His

P07356 (S2-D339)

12306  [NCBI]

STVHEILCKLSLEGDHSTPPSAYGSVKPYTNFDAERDALNIETAVKTKGVDEVTIVNILTNRSNVQRQDIAFAYQRRTKKELPSALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARELYDAGVKRKGTDVPKWISIMTERSVCHLQKVFERYKSYSPYDMLESIKKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD

Approximately 39.6 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, 4 mM DTT, pH 7.4 or 20 mM PBS, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.