ARF3 蛋白, Human (His)

ARF3 protein, a GTP-binding allosteric activator of cholera toxin, plays a role in protein trafficking and vesicle dynamics in the Golgi apparatus. Beyond toxin activation, ARF3 interacts with PRKCABP, participating in diverse cellular processes. It also associates with PI4KB and NCS1/FREQ at the Golgi complex, emphasizing its role in crucial molecular interactions for cellular functions. ARF3 Protein, Human (His) is the recombinant human-derived ARF3 protein, expressed by E. coli , with N-His labeled tag. The total length of ARF3 Protein, Human (His) is 181 a.a., with molecular weight of ~23 kDa.

ARF3 是一种 GTP 结合蛋白，充当霍乱毒素催化亚基（一种 ADP-核糖基转移酶）的变构激活剂。除了在毒素激活中的作用外，ARF3 还参与蛋白质运输，对高尔基体内囊泡出芽和脱壳产生潜在影响。这种多方面的蛋白质与 PRKCABP 相互作用，突出了它参与各种细胞过程。此外，ARF3 与高尔基复合体中的 PI4KB 和 NCS1/FREQ 相关，进一步强调其参与对细胞功能至关重要的分子相互作用。

Human

E. coli

N-His

P61204 (M1-K181)

377  [NCBI]

Approximately 23 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween80, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.