ART3 蛋白, Human (HEK293, His)

ART3 is a member of the mono-ADP-ribosyltransferase family genes which is involved in cell division, DNA-repair and the regulation of the inflammatory response. The temporary and restricted expression of ART3 in spermatocytes might be linked to cell division processes underlying the formation of spermatocytes and the regulation of spermatogenesis. Moreover, it induces TNBC cell growth and invasion by activating Akt and ERK. ART3 Protein, Human (HEK293, His) is the recombinant human-derived ART3 protein, expressed by HEK293 , with C-His labeled tag.

ART3 是单 ADP 核糖基转移酶家族基因的成员，该基因参与细胞分裂、DNA 修复和炎症反应的调节。ART3 mRNA 可在未刺激的单核细胞、肺泡上皮细胞、睾丸精母细胞、巨噬细胞、T 淋巴细胞和其他细胞类型中检测到。ART3的表达与生理和病理功能相关，包括精子发生和心肌细胞数量的损伤。ART3 在精母细胞中的临时和受限表达可能与精母细胞形成和精子发生调节的细胞分裂过程有关。并且ART3蛋白在人TNBC肿瘤组织和细胞系中显著过表达。MDA-MB-231 乳腺癌细胞中 ART3 的过度表达通过激活 Akt 和 ERK 增加细胞增殖、侵袭和体外存活以及异种移植肿瘤的生长。此外，ART3 可能在创伤性脑损伤 (TBI) 后的中枢神经系统 (CNS) 病理生理学中发挥重要作用^[1][2]。

Measured in a cell proliferation assay using MDA-MB-231 cells. The ED₅₀ for this effect is typically 1.039 μg/mL, corresponding to a specific activity is 962.464 units/mg.

Human

HEK293

C-6*His

AAH08397 (E27-S362)

419  [NCBI]

EVLDMADNAFDDEYLKCTDRMEIKYVPQLLKEEKASHQQLDTVWENAKAKWAARKTQIFLPMNFKDNHGIALMAYISEAQEQTPFYHLFSEAVKMAGQSREDYIYGFQFKAFHFYLTRALQLLRKPCEASSKTVVYRTSQGTSFTFGGLNQARFGHFTLAYSAKPQAANDQLTVLSIYTCLGVDIENFLDKESERITLIPLNEVFQVSQEGAGNNLILQSINKTCSHYECAFLGGLKTENCIENLEYFQPIYVYNPGEKNQKLEDHSEKNWKLEDHGEKNQKLEDHGVKILEPTQIPGMKIPEPFPLPEDKSQGNINNPTPGPVPVPGPKSHPSAS

Approximately 48-55 kDa due to the glycosylation.

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.