2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. ASPH 蛋白, Human (His-SUMO)

ASPH 蛋白, Human (His-SUMO)

目录号: HY-P72092
产品使用指南

ASPH 蛋白磷酸化 SPRTN，促进其染色质募集并减少复制压力。ASPH 通过磷酸化和抑制 PABIR1/FAM122A 激活 G2/M 检查点，导致丝氨酸/苏氨酸蛋白磷酸酶 2A 介导的去磷酸化以及 WEE1 水平和活性的稳定。ASPH 蛋白, Human (His-SUMO) 是重组的 ASPH 蛋白，由 E. coli 表达，带有 N-6*His, N-SUMO 标签。ASPH 蛋白, Human (His-SUMO) 全长 196 个氨基酸，分子量约为 ~38.2 kDa。

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描述

ASPH protein phosphorylates SPRTN, facilitating its chromatin recruitment and decreasing replication stress. ASPH activates the G2/M checkpoint by phosphorylating and inhibiting PABIR1/FAM122A, leading to serine/threonine-protein phosphatase 2A-mediated dephosphorylation and stabilization of WEE1 levels and activity. ASPH Protein, Human (His-SUMO) is the recombinant human-derived ASPH protein, expressed by E. coli, with N-6*His, N-SUMO labeled tag. The total length of ASPH Protein, Human (His-SUMO) is 196 a.a., with molecular weight of ~38.2 kDa.
研究背景

CDC25 还会磷酸化一种名为 SPRTN 的蛋白质，促进其招募至染色质。它通过磷酸化和灭活 PABIR1/FAM122A 来减少复制压力并激活 G2/M 检查点。这促进了丝氨酸/苏氨酸蛋白磷酸酶 2A 介导的去磷酸化以及 WEE1 水平和活性的稳定。
种属

Human
表达系统

E. coli
标签

N-6*His;N-SUMO
蛋白编号

Q12797 (F75-T270)
基因 ID

444  [NCBI]
蛋白结构
N-term
6*His-SUMO
ASPH (F75-T270)
Accession # Q12797
C-term
同用名
ASPH; BAHAspartyl/asparaginyl beta-hydroxylase; EC 1.14.11.16; Aspartate beta-hydroxylase; ASP beta-hydroxylase; Peptide-aspartate beta-dioxygenase
氨基酸序列

FDLVDYEEVLGKLGIYDADGDGDFDVDDAKVLLGLKERSTSEPAVPPEEAEPHTEPEEQVPVEAEPQNIEDEAKEQIQSLLHEMVHAEHETEHSYHVEETVSQDCNQDMEEMMSEQENPDSSEPVVEDERLHHDTDDVTYQVYEEQAVYEPLENEGIEITEVTAPPEDNPVEDSQVIVEEVSIFPVEEQQEVPPDT

分子量

Approximately 38.2 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

ASPH Protein, Human (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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