BamA 蛋白, E.coli (Myc, His)

BamA is a key member of the outer membrane protein assembly complex (Bam), which concentrates the assembly of β-barrel proteins into the outer membrane. Together with BamD, BamA forms the core mechanism of this process, which relies on the coordinated action of all five subunits. BamA Protein, E.coli (Myc, His) is the recombinant E. coli-derived BamA protein, expressed by E. coli , with N-10*His, C-Myc labeled tag.

BamA 是外膜蛋白组装复合物 (Bam) 的关键成分，在组装 β-桶蛋白并将其插入外膜的复杂过程中发挥着核心作用。BamA 与 BamD 一起构成了该组装过程的核心机制，并且底物的有效折叠和插入外膜需要所有五个亚基的协调作用。BamA 的结构特征包括带有侧门的 β 桶，该侧门在第一条链和最后一条链之间打开，有利于底物插入外膜。作为大肠杆菌菌株 EC93 的接触依赖性生长抑制效应器 CdiA-EC93 的受体，BamA 在介导这种微生物相互作用中至关重要。值得注意的是，它对 CdiA-EC93 的敏感性取决于存在的特定 BamA 变体，因为用不同细菌菌株的同源物替换 BamA 会改变敏感性。此外，BamA 在 CDI 中的作用似乎独立于 Bam 复合体的其他成分。

E.coli

E. coli

N-10*His;C-Myc

P0A940 (A175-G424)

944870  [NCBI]

AEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQKLAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGNLAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKTVKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRLGFFETVDTDTQRVPGSPDQVDVVYKVKERNTG

Approximately 36.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4 .

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.