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  3. BCAN 蛋白, Human (HEK293, His)

BCAN 蛋白, Human (HEK293, His)

目录号: HY-P7676
COA 产品使用指南

BCAN 蛋白, Human (HEK293, His)是在 HEK293 细胞中产生的重组人 Brevican 核心蛋白。在成人大脑中,brevican 核心蛋白经历蛋白水解裂解,以145kda 的全长形式和 80kda 的 C 末端片段存在。

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规格 价格 是否有货 数量
10 μg ¥1150 In-stock
50 μg ¥3450 In-stock
100 μg   询价  

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  • 参考文献

描述

BCAN Protein, Human (HEK293, His) is a recombinant human brevican core protein produced in HEK293 cells. In the adult brain, the brevican core protein undergoes proteolytic cleavage and exists as a 145-kDa full-length form and an 80-kDa C-terminal fragment[1].

研究背景

Brevican 是蛋白聚糖聚集蛋白聚糖/多功能蛋白聚糖家族的成员,其 N 末端含有透明质酸结合结构域,C 末端含有凝集素样结构域。Brevican 是该家族中最小的核心蛋白,也是成人大脑中最丰富的硫酸软骨素蛋白聚糖之一[2]

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q96GW7-1 (D23-P911)

基因 ID
蛋白结构
N-term
BCAN (D23-P911)
Accession # Q96GW7-1
6*His
C-term
中文名
重组人 Brevican 核心蛋白, His (HEK293 表达)
同用名
rHuBCAN, His; Brevican Core Protein; BCAN; BEHAB;
氨基酸序列

DVLEGDSSEDRAFRVRIAGDAPLQGVLGGALTIPCHVHYLRPPPSRRAVLGSPRVKWTFLSRGREAEVLVARGVRVKVNEAYRFRVALPAYPASLTDVSLALSELRPNDSGIYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFSGAQEACARIGAHIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQTPREACYGDMDGFPGVRNYGVVDPDDLYDVYCYAEDLNGELFLGDPPEKLTLEEARAYCQERGAEIATTGQLYAAWDGGLDHCSPGWLADGSVRYPIVTPSQRCGGGLPGVKTLFLFPNQTGFPNKHSRFNVYCFRDSAQPSAIPEASNPASNPASDGLEAIVTVTETLEELQLPQEATESESRGAIYSIPIMEDGGGGSSTPEDPAEAPRTLLEFETQSMVPPTGFSEEEGKALEEEEKYEDEEEKEEEEEEEEVEDEALWAWPSELSSPGPEASLPTEPAAQEESLSQAPARAVLQPGASPLPDGESEASRPPRVHGPPTETLPTPRERNLASPSPSTLVEAREVGEATGGPELSGVPRGESEETGSSEGAPSLLPATRAPEGTRELEAPSEDNSGRTAPAGTSVQAQPVLPTDSASRGGVAVVPASGDCVPSPCHNGGTCLEEEEGVRCLCLPGYGGDLCDVGLRFCNPGWDAFQGACYKHFSTRRSWEEAETQCRMYGAHLASISTPEEQDFINNRYREYQWIGLNDRTIEGDFLWSDGVPLLYENWNPGQPDSYFLSGENCVVMVWHDQGQWSDVPCNYHLSYTCKMGLVSCGPPPELPLAQVFGRPRLRYEVDTVLRYRCREGLAQRNLPLIRCQENGRWEAPQISCVPRRPARALHPEEDPEGRQGRLLGRWKALLIPPSSPMPGPHHHHHH

分子量

75-150 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against 20 mM PB,150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

BCAN Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BCAN Protein, Human (HEK293, His)
目录号:
HY-P7676
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