BCAS2 蛋白, Human (His, T7)

BCAS2 Protein, Human (His, T7) plays crucial roles in pre-mRNA splicing and androgen receptor transcription. BCAS2 helps repair radiation-induced DSBs efficiently in both human PCa cells and Drosophila^[1][2].

BCAS2 是一种在晚期前列腺癌中富含的蛋白质，与 NBS1 相互作用以增强 DNA 双链断裂修复。BCAS2 可能通过与 NBS1 相互作用来增强 NHEJ 和 HR，其中涉及 BCAS2 N 端以及 NBS1 N 端和 C 端。BCAS2 过度表达与 PCa 患者较高的格里森分级和病理分级以及较短的生存期显着相关^[2]。

Human

E. coli

C-6*His;N-T7

O75934 (A2-F225)

10286  [NCBI]

ASMTGGQQMGAGTGLVAGEVVVDALPYFDQGYEAPGVREAAAALVEEETRRYRPTKNYLSYLTAPDYSAFETDIMRNEFERLAARQPIELLSMKRYELPAPSSGQKNDITAWQECVNNSMAQLEHQAVRIENLELMSQHGCNAWKVYNENLVHMIEHAQKELQKLRKHIQDLNWQRKNMQLTAGSKLREMESNWVSLVSKNYEIERTIVQLENEIYQIKQQHGEANKENIRQDF

Approximately 28 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 20 mM Tris-HCl, 200 mM NaCl, 2 mM DTT, pH 8.0 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Li-Po Wang, et al. BCAS2, a protein enriched in advanced prostate cancer, interacts with NBS1 to enhance DNA double-strand break repair. Br J Cancer. 2020 Dec;123(12):1796-1807.  [Content Brief]

  [2]. Ángel Salmerón-Hernández, et al. BCAS2 Enhances Carcinogenic Effects of Estrogen Receptor Alpha in Breast Cancer Cells. Int J Mol Sci. 2019 Feb 22;20(4):966.  [Content Brief]