BCAS2 蛋白, Human (N-GST)

BCAS2 is essential for pre-mRNA splicing and is critical for spliceosome activation as a key component of the activated spliceosome and the PRP19-CDC5L complex. It may act as a scaffold in spliceosome assembly, forming contacts with core components. BCAS2 Protein, Human (N-GST) is the recombinant human-derived BCAS2 protein, expressed by E. coli , with N-GST labeled tag. The total length of BCAS2 Protein, Human (N-GST) is 224 a.a., with molecular weight of ~53 kDa.

BCAS2 对于前 mRNA 剪接至关重要，是激活剪接体和 PRP19-CDC5L 复合物的重要组成部分，而 PRP19-CDC5L 复合物是剪接体激活不可或缺的一部分。它通过与所有其他核心复杂组件建立联系，在剪接体组装中发挥潜在的支架作用。BCAS2 是 PRP19-CDC5L 复合体的一部分，也可能与 DNA 损伤反应 (DDR) 有关。BCAS2 存在于催化前和催化剪接体复合物以及催化后剪接体 P 复合物中。PRP19-CDC5L 剪接复合体以 PRPF19、CDC5L、PLRG1 和 BCAS2 的同源四聚体为核心，还包括不太稳定的相关蛋白，如 CTNNBL1、CWC15 和 HSPA8。BCAS2 直接与该复合物中的 PRPF19、CDC5L 和 PLRG1 相互作用。

Human

E. coli

N-GST

O75934 (A2-F225)

10286  [NCBI]

Approximately 53 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of Tris-based buffer, 50% glycerol

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.