1. 重组蛋白
  2. Enzymes & Regulators
  3. Protein Tyrosine Kinases
  4. Brutons Tyrosine Kinase (BTK)
  5. BTK 蛋白, Human (Sf9, His)

BTK 蛋白, Human (Sf9, His)

目录号: HY-P72048
COA 产品使用指南

BTK 蛋白是一种关键的非受体酪氨酸激酶,对于 B 淋巴细胞的发育和信号传导至关重要。BCR激活后,BTK启动级联反应,磷酸化PLCG2并激活下游通路,影响钙动员和PKC激活。BTK 蛋白, Human (Sf9, His) 是重组的 BTK 蛋白,由 Sf9 insect cells 表达,带有 N-10*His 标签。

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规格 价格 是否有货 数量
20 μg ¥2150 In-stock
50 μg ¥4350 In-stock
100 μg ¥7000 询价
> 100 μg   询价  

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描述

The BTK protein is a key non-receptor tyrosine kinase that is essential for B lymphocyte development and signaling. After BCR activation, BTK initiates a cascade reaction, phosphorylates PLCG2 and activates downstream pathways, affecting calcium mobilization and PKC activation. BTK Protein, Human (Sf9, His) is the recombinant human-derived BTK protein, expressed by Sf9 insect cells , with N-10*His labeled tag.

研究背景

BTK 蛋白是一种非受体酪氨酸激酶,对于 B 淋巴细胞的发育、分化和信号传导是不可或缺的。抗原与 B 细胞抗原受体 (BCR) 结合后,BTK 会触发信号级联,导致 B 细胞激活、磷酸化 PLCG2 并启动涉及钙动员和蛋白激酶 C (PKC) 激活的下游途径。BTK 作为多种信号蛋白的平台,参与细胞因子受体信号通路,在先天性和适应性免疫中至关重要,特别是在 Toll 样受体 (TLR) 通路中。它在调节脾 B 细胞中的 TLR9 激活中发挥着关键作用,并诱导 NF-kappa-B 的活性,从而有助于众多基因的转录调节。此外,BTK 还能激活 NLRP3 炎症小体组装、磷酸化转录因子 GTF2I,并参与细胞凋亡调节,凸显其在免疫反应和细胞过程中的多方面功能。

种属

Human

表达系统

Sf9 insect cells

标签

N-10*His

蛋白编号

Q06187 (M1-S659)

基因 ID

695  [NCBI]

蛋白结构
N-term
10*His
BTK (M1-S659)
Accession # Q06187
C-term
同用名
Agammaglobulinaemia tyrosine kinase; AGMX 1; AGMX1; AT; ATK; B cell progenitor kinase; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton tyrosine kinase; Bruton Tyrosine Kinase; Btk; BTK_HUMAN; dominant-negative kinase-deficient Brutons tyrosine kinase; IMD 1; IMD1; MGC126261; MGC126262; OTTHUMP00000063593; PSCTK 1; PSCTK1; truncated Bruton agammaglobulinemia tyrosine kinase; Tyrosine protein kinase BTK; Tyrosine-protein kinase BTK; tyrosine-protein kinase BTK isoform lacking exon 14; XLA
氨基酸序列

MAAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVVPEKNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKKPLPPEPAAAPVSTSELKKVVALYDYMPMNANDLQLRKGDEYFILEESNLPWWRARDKNGQEGYIPSNYVTEAEDSIEMYEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKSTGDPQGVIRHYVVCSTPQSQYYLAEKHLFSTIPELINYHQHNSAGLISRLKYPVSQQNKNAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGKWRGQYDVAIKMIKEGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERPTFKILLSNILDVMDEES

分子量

Approximately 78.3 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm sterile filtered 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BTK Protein, Human (Sf9, His)
目录号:
HY-P72048
需求量: