C1QTNF1/CTRP1 蛋白, Human (HEK293, His)

C1QTNF1/CTRP1 Protein, Human (HEK293, His) is a recombinant human C1q/TNF-related protein 1 (CTRP1) produced in HEK293 cells, with His tag. CTRP1 is an adipokine that is mainly expressed in adipose-derived cells and adipocytes^[1].

CTRP1 在调节身体能量稳态和胰岛素敏感性方面发挥着重要作用。高水平的循环 CTRP1 与代谢综合征、充血性心脏病和高血压有关^[1]。

1. Measured by its binding ability in a functional ELISA. Immobilized Human BAI3(HY-P7675) at 2 μg/mL or 4 μg/mL (100 μL/well) can bind Biotinylated Human C1qTNF1. The ED₅₀ for this effect is ≤10 µg/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized Human BAI3(HY-P7675) at 4μg/mL (100μ l/well) can bind Human CTRP1. The ED₅₀ for this effect is 2.897μg/mL.

• 
  Measured by its binding ability in a functional ELISA. Immobilized Human BAI3 at 2 μg/mL (100 μL/well) can bind Biotinylated Human C1qTNF1, The ED₅₀ for this effect is 1.533 µg/mL.

Human

HEK293

C-6*His

Q9BXJ1-1 (R26-P281)

114897  [NCBI]

RVPHVQGEQQEWEGTEELPSPPDHAERAEEQHEKYRPSQDQGLPASRCLRCCDPGTSMYPATAVPQINITILKGEKGDRGDRGLQGKYGKTGSAGARGHTGPKGQKGSMGAPGERCKSHYAAFSVGRKKPMHSNHYYQTVIFDTEFVNLYDHFNMFTGKFYCYVPGLYFFSLNVHTWNQKETYLHIMKNEEEVVILFAQVGDRSIMQSQSLMLELREQDQVWVRLYKGERENAIFSEELDTYITFSGYLVKHATEPHHHHHH

Approximately 35-40 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.2-7.4 or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Joanna Dorota Janowska. C1q/TNF-related Protein 1, a Multifunctional Adipokine: An Overview of Current Data. Am J Med Sci. 2020 Sep;360(3):222-228.  [Content Brief]

  [2]. Linhui Shen, et al. Association of C1q/TNF-related protein-1 (CTRP1) serum levels with coronary artery disease. J Int Med Res. 2019 Jun; 47(6): 2571-2579.  [Content Brief]